Seribu Hari Pertama Kehidupan (1000 HPK) merupakan fase kehidupan yang sangat penting dalam upaya pencegahan stunting. Berdasarkan data UNICEF (United Nations Children's Fund) tahun 2019, sebanyak 21,3% anak mengalami stunting, khususnya di Kabupaten Lampung Tengah sebanyak 1420 balita, di Wilayah Kerja Puskesmas Candi Rejo. Balita tersebut termasuk dalam kategori sangat pendek sebanyak 107 orang dan balita pendek sebanyak 263 orang. Upaya penurunan stunting yang dilakukan adalah dengan memberikan promosi kesehatan kepada calon pengantin terkait fase 1000 HPK balita. Tujuan penelitian adalah untuk mengetahui pengaruh promosi kesehatan terhadap pengetahuan calon pengantin tentang 1000 hari pertama kehidupan di Wilayah Kerja Puskesmas Candi Rejo, Kecamatan Way Pengubuan, Kabupaten Lampung Tengah tahun 2022.Penelitian kuantitatif menggunakan pendekatan one group pretest-postest. Populasi penelitian ini adalah seluruh calon pengantin dengan sampel sebanyak 31 orang secara total sampling dengan pendekatan one group pretest-postest. Penelitian dilakukan di Wilayah Kerja Puskesmas Candi Rejo, Kecamatan Way Pengubuan, Kabupaten Lampung Tengah pada bulan Juli 2022. Pengumpulan data menggunakan lembar observasi.Hasil rerata pengetahuan calon pengantin tentang 1000 hari pertama kehidupan sebelum diberikan promosi kesehatan adalah 35,3 dan setelah diberikan promosi kesehatan adalah 95,9. Pengaruh promosi kesehatan terhadap pengetahuan calon pengantin tentang 1000 hari pertama kehidupan di Wilayah Kerja Puskesmas Candi Rejo, Kecamatan Way Pengubuan, Kabupaten Lampung Tengah tahun 2022 menunjukkan nilai p = 0,000. Saran: Bagi tenaga kesehatan, diharapkan untuk lebih meningkatkan edukasi yang diberikan berupa leaflet kepada calon pengantin tentang pentingnya 1000 hari pertama kehidupan. Kata Kunci : 1000 hari pertama kehidupan, promosi kesehatan, pengetahuan, calon pengantin ABSTRACT The First Thousand Days of Life (1000 HPK) is a very important phase of life in stunting prevention efforts. Based on UNICEF (United Nations Children's Fund) data in 2019, as many as 21,3% of children are stunted, particularly in Central Lampung Regency as many as 1420 toddlers, in the Working Area of the Candi Rejo Health Centre. The toddler is included in the very short category as many as 107 people and short toddlers as many as 263 people. The efforts to reduce stunting are providing health promotion to brides and grooms related to the 1000 HPK toddler phase. The purpose of the study is to determine the effect of health promotion on the bride and groom's knowledge about the first 1000 days of life in the Working Area of the Candi Rejo Health Center, Way Pengubuan District, Central Lampung Regency in 2022.Quantitative research was used with one group pretest-postest approach. The population of this study is the entire bride and groom with a sample used as many as 31 people in total sampling by means of one group pretest-postest. The study was conducted in the Working Area of the Candi Rejo Health Center, Way Pengubuan District, Central Lampung Regency in July 2022. Data collection used observation sheet.The average results of the bride and groom knowledge about the first 1000 days of life before given health promotion was 35.3 and after given health promotion was 95.9. The effect of health promotion on the knowledge of the bride and groom about the first 1000 days of life in the Working Area of the Candi Rejo Health Center, Way Pengubuan District, Central Lampung Regency in 2022 showed a p-value = 0.000. Suggestion: For health workers, it is expected to further improve the education given in the form of leaflets to the bride and groom on the importance of 1000 days of life. Keywords : first 1000 days, health promotion, knowledge, bride and groom
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