EKSTRAK ETANOLIK DAUN AWAR–AWAR (Ficus septica Burm. f.) MEMACU APOPTOSIS SEL KANKER PAYUDARA MCF-7 MELALUI PENEKANAN EKSPRESI Bcl-2

Dewi Arum Sekti (Faculty of Pharmacy, Universitas Gadjah Mada)
Muhammad Fithrul Mubarok (Faculty of Pharmacy, Universitas Gadjah Mada)
Inna Armandani (Faculty of Pharmacy, Universitas Gadjah Mada)
Sendy Junedy (Faculty of Pharmacy, Universitas Gadjah Mada)
Edy Meiyanto (Faculty of Pharmacy, Universitas Gadjah Mada)

Majalah Obat Tradisional
Vol 15, No 3 (2010)

Alkaloid fenantroindolisidin showed cytotoxic activity against several cancer cells. Ethanolic leaf extract of Awar-awar proven to increase the cytotoxic activity of chemotherapy agent doxorubicin in MCF-7 cells. This study aims to determine the effect of Ethanolic leaf extract of Awar-awar in apoptosis induction of breast cancer cells MCF-7.Awar-awar leaf powder was extracted by maceration using 70% ethanol and then concentrated to obtain concentrated of Awar-awar. Apoptosis induction was examined by double staining method and the expression of Bcl-2 protein was observed by  immunohistochemistry method. Single treatment of Awar-awar leaf extract  showed the ability of extracts to induce apoptosis and downregulated the expression of Bcl-2 protein. Ethanolic leaf extract of Awar-awar is potential as co-chemotherapeutic agent.