This research aims to develop and validate a new instrument for measuring chemistry teacher’s self-efficacy for differentiated instruction. Data were collected using questions developed based on a literature study. The data were analyzed using exploratory factor analysis (EFA), confirmatory factor analysis (CFA), and correlational techniques on responses from who participated in an offline and online survey. The findings from EFA, CFA, and correlation analysis provide sufficient empirical evidence to support the convergent and discriminant validity of the instrument. The high Cronbach’s coefficient alpha values show the instrument’s good internal consistency and reliability. We documented a valid and reliable measure of chemistry teachers’ self-efficacy for differentiated instruction, comprising six constructs with 22 items, by integrating evidence from theory and data. The six-factor scale is named "efficacy in differentiating the learning process, efficacy in differentiating learning content, efficacy in differentiating learning products, efficacy in identifying student differences, efficacy in assessment, and efficacy in differentiating the learning environment". This instrument enriches the theoretical understanding of teacher self-efficacy in differentiated instruction by providing a domain-specific measurement model tailored for chemistry education. Practically, the scale offers teacher educators, policymakers, and professional development programs a diagnostic tool to identify teachers’ strengths and areas needing support, enabling more targeted training interventions to enhance differentiated instruction in classroom practice.
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