JURNAL ATOMIK
Vol 10 No 2 (2025)

SELEKSI AMILASE PENDEGRADASI PATI MENTAH BAKTERI DARI AIR GALIAN BEKAS TAMBANG BATU BARA DI DESA LOA BAHU

Akbar, Akbar (Unknown)
Astuti, Winni (Unknown)
Pratiwi, Djihan Ryn (Unknown)



Article Info

Publish Date
29 Aug 2025

Abstract

Raw starch-degrading amylase (RSDA) is a type of hydrolase enzyme that can degrade raw starch into glucose without going through the heating and gelatinization process. This study was conducted to select RSDA-producing bacteria from ex-coal mine water samples in Loa Bahu Village, Sungai Kunjang, Samarinda, with various types of raw starch such as rice, wheat, corn, potatoes, sago, and cassava. The selection of bacteria-producing RSDA enzymes was carried out using agar nutrient media containing raw starch, the positive test was marked by the formation of a clear zone around the bacterial colony after the addition of iodine solution. A total of 13 bacterial isolates (AT 1 - AT 13) were tested qualitatively by RSDA. The selection results showed that 4 bacterial isolates were capable of degrading raw rice starch, 4 bacterial isolates were capable of degrading raw corn starch, 2 bacterial isolate was capable of degrading raw cassava starch, 1 bacterial isolate was capable of degrading raw potato starch, 1 isolate was capable of degrading raw wheat starch, and 2 bacterial isolates were able to degrade raw sago starch. The bacterial isolate with the code AT3 is capable of degrading raw starch of rice, corn, potatoes, wheat and sago.

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Journal Info

Abbrev

JA

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

Description

Jurnal Atomik (JA) is published twice a year (March and August) by Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University. Jurnal Atomik is a media for graduate students of Department of Chemistry to publish their research articles in Chemistry field including ...