Journal of Biodiversity and Biotechnology
Vol 4, No 1 (2024)

Karyotype Analysis of Phalaenopsis Hibryds with Colchicine Induction

Sri Hartati (Departement of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret [SCOPUS Author ID: 57193731751])
Samanhudi Samanhudi (Departement of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret)
Hasna Afanin (Unknown)



Article Info

Publish Date
07 Aug 2025

Abstract

Phalaenopsis hybrid is one of the orchids that is very important in the horticultural market as an ornamental plant whose demand continues to increase. This causes the need for breeding in order to obtain new varied flower characters as desired by polyploidization. Polyploidy induction in Phalaenopsis hybrids was carried out using colchicine. The purpose of this study was to determine the karyotype pattern of Phalaenopsis hybrids. The research was conducted at the Screenhouse of the Faculty of Agriculture and Integrated Laboratory Unit of Sebelas Maret University, Surakarta in August 2021-March 2022. Polyploidy induction was carried out by dripping 1500 ppm concentration of colchicine on flower bud of Phalaenopsis hybrid. The method used in this research is squashing. The experimental results showed that the karyotype pattern in control Phal. Golden Tree was 2n=3x-7=50m, while in Phal. Fuller Sunset and Phal. OX X-ray was 2n=4x=76m. The karyotype pattern in orchids with colchicine induction was Phal. Golden Tree 2n=6x-14=100m, while in Phal. Fuller Sunset and Phal. OX X-ray 2n=8x=152m. 

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Journal Info

Abbrev

jbb

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, ...