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All Journal Agrosains: Jurnal Penelitian Agronomi Journal of Biodiversity and Biotechnology
Sri Hartati
Departement of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret [SCOPUS Author ID: 57193731751]
Agriculture, Biological Sciences & Forestry

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Penambahan Bahan Organik dan NAA terhadap Pertumbuhan Anggrek Hasil Persilangan Coelogyne secara In Vitro Sri Hartati; Sukaya Sukaya; Dwi Alma Indah Puji Astuti
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 2 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i2.79509

Abstract

Orchids are ornamental plants that have a high selling value so they are very prospective in the world of the ornamental plant business. This high economic value means that market demand for orchid commodities tends to increase. Efforts made to meet the need for orchids in the market are by propagating orchids using in vitro culture. This research aims to determine the effect of adding organic materials and NAA on the growth of orchids resulting from crossing Coelogyne pandurata and Coelogyne rumphii in vitro. This research used a completely randomized design (CRD) with two factors, namely the type of organic material (tomato, carrot and potato) and the concentration of ZPT NAA (0, 1 and 2 ppm). The research results showed that the addition of potato and tomato organic materials to the VW base medium was able to stimulate plant growth. The added NAA concentrations of 1 and 2 ppm are able to stimulate optimal plant growth. The combination of adding tomato organic matter and 1 ppm NAA concentration had the best growth in the number of roots and leaves, while adding tomato organic matter and 2 ppm NAA had the best growth in the number of shoots. The combination of adding potato organic material and 2 ppm NAA can stimulate plant height growth.
Effect of Auxin and Cytokinin on Orchid Subculture Resulting from Coelogyne Crosses Sri Hartati; Ahmad Yunus; Iistikharoh Iistikharoh
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.80626

Abstract

The black orchid or Coelogyne is one of the native orchids of Kalimantan, widely cultivated in Indonesia. Several species of Coelogyne orchids have been crossed to obtain new superior-quality plants. Modifying the media, such as giving PGR, is necessary for good plant multiplication results. This research was conducted by changing MS growing media with ZPT BA and NAA. This experiment aimed to determine the effect of ZPT BA and NAA on the growth of orchid subcultures from a cross between Coelogyne pandhurata and Coelogyne rumphii. The study used a completely randomized design (RAL) with 2 factors, namely ZPT BA and NAA, each having a concentration of 0 ppm, 0.2 ppm; 0.4 ppm, and 0.6 ppm with 16 treatment combinations and 4 replications. They were implemented at the Laboratory Plant Physiology and Biotechnology, Faculty of Agriculture, UNS Surakarta, from November 2020 to March 2021. The variables observed included the number of shoots, plant length, plant weight, number of leaves, number of roots, root length, when roots appeared, and when shoots appeared. The results showed that BA 0.6 ppm gives the best output on the growth of the number of shoots, and NAA concentration of 0,2 ppm gives the best results on the variable number of roots. The combination of BA 0.6 ppm and NAA 0.2 ppm gave the best results on plant weight growth.
Morphological Character of The Natural Orchid Phalaenopsis Spp. Results of Colchicine Polyploidy Induction Sri Hartati; Samanhudi Samanhudi; Endang Suprapti
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108518

Abstract

Induction of polyploidy in orchids can cause morphological changes such as larger leaf and flower sizes, longer bloom durations, and sharper flower colors. Morphological characterization needs to be done to determine the morphological changes that occur after colchicine treatment. This study aims to determine the morphological characters of natural orchids Phalaenopsis spp. polyploidy induction using colchicine. The research was carried out from November 2021-February 2022 at the Screen House, Faculty of Agriculture, UNS. The plant materials used included P. violacea from Sulawesi, P. violacea from Java, and P. Ambilis from Ambon. The treatment used was colchicine 1,250 ppm which was applied to flower buds using the cotton method with 2 replications. The collected data were analyzed by descriptive analysis and paired T-test using IBM SPSS Statistics 26 to compare the average performance of mutant plants with control plants. Induction of polyploidy using colchicine with a concentration of 1,250 ppm could increase the size of leaves, flower stalks, flowers, dorsal and lateral sepals, and petals in P. violacea from Java, P. violacea from Sulawesi, and P. amabilis from Ambon.
Karyotype Analysis of Phalaenopsis Hibryds with Colchicine Induction Sri Hartati; Samanhudi Samanhudi; Hasna Afanin
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.101961

Abstract

Phalaenopsis hybrid is one of the orchids that is very important in the horticultural market as an ornamental plant whose demand continues to increase. This causes the need for breeding in order to obtain new varied flower characters as desired by polyploidization. Polyploidy induction in Phalaenopsis hybrids was carried out using colchicine. The purpose of this study was to determine the karyotype pattern of Phalaenopsis hybrids. The research was conducted at the Screenhouse of the Faculty of Agriculture and Integrated Laboratory Unit of Sebelas Maret University, Surakarta in August 2021-March 2022. Polyploidy induction was carried out by dripping 1500 ppm concentration of colchicine on flower bud of Phalaenopsis hybrid. The method used in this research is squashing. The experimental results showed that the karyotype pattern in control Phal. Golden Tree was 2n=3x-7=50m, while in Phal. Fuller Sunset and Phal. OX X-ray was 2n=4x=76m. The karyotype pattern in orchids with colchicine induction was Phal. Golden Tree 2n=6x-14=100m, while in Phal. Fuller Sunset and Phal. OX X-ray 2n=8x=152m. 
Co-Authors Ahmad Yunus Dwi Alma Indah Puji Astuti Endang Suprapti Hasna Afanin Iistikharoh Iistikharoh Samanhudi Samanhudi Sukaya Sukaya