This study aimed to evaluate the potential of the entomopathogenic fungus Beauveria bassiana as an endophytic agent through soil drenching application and to assess its effect on the growth of yardlong bean plants (Vigna unguiculata subsp. sesquipedalis). The research was conducted under both in vitro and in vivo conditions using a Completely Randomized Design (CRD) with four conidial density treatments: control (distilled water), 1 × 10⁸, 1 × 10⁷, and 1 × 10⁶ conidia/mL, each replicated five times. Data were analyzed using ANOVA followed by Tukey’s Honest Significant Difference (HSD) test at a 5% significance level. The results demonstrated that B. bassiana successfully colonized plant tissues, with the highest colonization rate observed at 1 × 10⁸ conidia/mL, reaching 83.3% in roots and 66.7% in stems. Although differences in conidial density did not consistently produce significant effects on plant height and leaf number across all observation periods, inoculated treatments tended to show higher average growth values compared to the control, particularly at 28 days after planting. It can be concluded that soil application of B. bassiana is effective in establishing endophytic colonization without inhibiting vegetative growth, indicating its potential as an environmentally friendly pest management strategy.
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