Differentiated learning is an approach that provides space for students to learn according to their needs, interests, and learning profiles. However, in practice, the application of differentiated learning in elementary schools, especially in science subjects (Natural and Social Sciences), is not easy. This study aims to identify and analyze the challenges faced by teachers in the implementation of differentiated learning at SDN Karangroto 02. Using a descriptive qualitative approach, data is collected through observation, interviews, and documentation. The results of the study show that the main challenges include limited planning time, lack of teachers' in-depth understanding of the concept of differentiation, high administrative burden, and limited learning resources and facilities. In addition, the diversity of student characteristics and the level of readiness to learn are also inhibiting factors. This study recommends continuous training for teachers, adequate provision of resources, and stronger collaboration between education stakeholders to support the successful implementation of differentiated learning
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