Thrive Health Science Journal
Vol. 3 No. 1 (2026): March

Determination of Total Flavonoid Content of Ashitaba (Angelica keiskei Koidz.) Leaf Extract Using UV-Vis Spectrophotometry

Andriani, Baiq Yunita (Unknown)
Rahayu, Supiani (Unknown)
Rahayu, Lale Budi Hutami (Unknown)



Article Info

Publish Date
30 Mar 2026

Abstract

Ashitaba (Angelica keiskei Koidz.) is a medicinal plant known to be rich in active flavonoid compounds, particularly chalcones, which exhibit antioxidant, anti-inflammatory, and antidiabetic activities. This study aimed to determine the total flavonoid content in Ashitaba leaf extract obtained from Sembalun Village using UV-Vis spectrophotometry. The extract was obtained by maceration using 96% ethanol. Quantitative analysis was performed at the maximum wavelength of 415 nm, with quercetin as the standard. Calibration curves were prepared using quercetin concentrations of 20, 30, 40, 50, and 60 ppm on the X-axis, with absorbance values of 0.297, 0.337, 0.436, 0.509, and 0.588 on the Y-axis, resulting in the regression equation Y = 0.0094x + 0.0472 with a correlation coefficient (r) of 0.9677, indicating a linear relationship between concentration and absorbance. Absorbance values of the sample measured in triplicate were 0.661, 0.634, and 0.676, yielding an average total flavonoid content of 25.948 mgQE/g in Ashitaba leaf extract.

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Journal Info

Abbrev

thrive

Publisher

Subject

Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

Description

THRIVE Health Science Journal (THSJ) publishes original research findings and review articles related to Public Health, Medicine and Sciences. Papers presented at recognized conferences may be considered for publication. THSJ is an Open Access Journal where readers have access to full text for free ...