<![CDATA[Heavy metal contamination, particularly by copper and lead, in aquatic ecosystems poses a significant threat to ecological balance, aquatic organisms, microbial diversity, and human health. Therefore, the exploration of bacteria capable of withstanding high levels of copper and lead is crucial for the development of effective bioremediation agents. This study aimed to isolate and characterize bacterial strains resistant to copper and lead from sediment samples collected from the Kali Baru estuary in Semarang. Bacterial isolation was conducted using a serial dilution technique up to 10?², performed in triplicate. Emerging bacterial colonies were purified and subsequently subjected to macroscopic and microscopic morphological characterization. Resistance profiling of the isolates was assessed by exposing them to copper and lead at concentrations of 100, 200, and 300 mg/L. A total of 32 bacterial isolates were obtained from the sediment samples. Among these, two isolates demonstrated resistance to copper and 14 isolates exhibited resistance to lead at the highest concentration tested (300 mg/L). These metal-tolerant bacterial isolates show promising potential for application in bioremediation strategies targeting environments contaminated with copper and lead.]]>
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