Article Per Year (5 Year)
p-Index From 2020 - 2025
1.908
P-Index
This Author published in this journals
All Journal Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Jurnal Akademika Biologi Jurnal Biologi Universitas Andalas Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi Advance Sustainable Science, Engineering and Technology (ASSET)
Arina Tri Lunggani
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Education Electrical & Electronics Engineering Energy Environmental Science Materials Science & Nanotechnology Other

Published : 24 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 24 Documents
Search

 1   2   3 
Phylogenetic Analysis Of Pigmented Marine Derived Yeast Associated With Sargassum sp. Based On Internal Transcribed Spacer (ITS) Tri Lunggani, Arina; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.484 KB) | DOI: 10.14710/bioma.21.1.55-59

Abstract

Karimunjawa Jepara is a region with high diversity, including the diversity of seaweed. Brown seaweed is potential marine organisms due to their ability to produce enzymes, pigments, and bioactive compounds. This ability makes brown seaweed one of the potential biological agents from the marine to be developed in the industrial field. However, most of these substances may not be produced by seaweed itself, but cooperation with microbes or even by bacteria or symbiotic fungi. Secondary metabolites which are pharmaceutical, enzyme, and cosmetic sources can be produced by microbial associan. The purpose of this study was to carry out phylogenetic analysis and morphological characterization of colonies from pigmented yeast associated with Sargassum sp. The results of the phylogenetic analysis indicate that isolates of KY 3 have 100% relative similarity with Cystobasidium oligophagum.
Chromosomes and Mitotic Cell Division Phase In Onion Roots After 24 Hours Acetoorcein Soaking Time Kusumaningrum, Hermin Pancasakti; Lunggani, Arina Tri; Nurhakim, Muhammad Amal
Bioma : Berkala Ilmiah Biologi Vol. 14, No. 2, Tahun 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.889 KB) | DOI: 10.14710/bioma.14.2.46-48

Abstract

Onions (Allium cepa) are usually used in vitro to assess effect of chemical subtances by allowing developing roots to come into contact with substances to be tested. Acetic orcein staining of onion chromosomes has remained a standard method of preparation. However, aceto-orcein stain is corrosive and poisonous chemical substances since it containing oxidising agents such as organic peroxides, the toxic substances which are are cyanides, acid corrosives agents, and  also radioactive substances.  This research study mitotic activity in the roots of onion plants to determine the effects of soaking time of aceto orcein dye on actively dividing root cells. A series of several root tip from each bulb was harvested were soaked in 1, 3 and 24 hours on aceto-orcein stain and processed further for cytological studies by the aceto-orcein squash technique. The research  was carried out to study the effect of to mitotic index and chromosomal aberration  on  onion root. It will determine the percentage of cells that are undergoing mitosis. The squash techniques were used to observe mitosis in the tip of onion root cells during actively mitotic division cells time. Mitotic divisions occur in several phases, consist of prophase, metaphase, anaphase, telophase and interphase. Experiment were repeated six times for every soaking time. The data was analyzed by using T-Test. The result showed that various duration of soaking time significantly influenced the  reduction of mitotic index value. The lowest mitotic index  on glyphosate concentration 100 ppm i.e. 10. 73% and 7.19% for the duration  of soaking time 3 and 6 hours. The highest mitotic index on  glyphosate concentration 0 ppm i.e.  37.71% and 32.76% for the duration  of soaking time 3 and 6 hours. The result also showed that the chromosomal aberration were increased significantly. The lowest  chromosomal aberration  obtained i.e. 2.55% and 2.96% for the duration of aceto orcein soaking time 1, 3 and 24 hours. The highest chromosomal aberration obtained i.e. 21.71% and 36.26% for the duration of soaking time 1,3 and 24 hours. The type of chromosomal aberration were abnormal prophase, stickiness, bridge, abnormal anaphase, clumping chromosome, c- metaphase,  change of nucleous  shape and size. At 72h, their cytotoxic effects on the root tips showed strong growth retardation in high concentrations of all the wastewaters. Compared to the control, treatment with the wastewaters resulted in root growth inhibition with EC50 values of 35, 50 and 62% for bottling, rubber and brewery effluents respectively, and decrease in mitotic index with increasing concentration for all samples and these were statistically significant (p<0.05). Chromosomal aberrations induced in the onion root tip cells were mostly sticky chromosomes and bridges. Chromosomes with disturbed spindles and fragments were also present in appreciable amounts. Based on the EC50 values, the bottling wastewater was most toxic, followed by rubber effluent while effluents from the brewery were least toxic. The findings in this study indicate that there are toxic chemicals present in the wastewaters which are responsible for the observed genotoxic effects on the onion root tip cells. The study also reveals that the Allium test is a useful and reliable tool for the genotoxicity screening of industrial effluents which could be employed by environmental managers before these effluents are finally discharged into the environment.     Key words: chromosomes, onion roots, acetoorcein
Kemampuan Bakteri Asam Laktat Dalam Menghambat Pertumbuhan dan Produksi Aflatoksin B2 Aspergilllus flavus Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 09, No. 2, Tahun 2007
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.061 KB) | DOI: 10.14710/bioma.9.2.45-51

Abstract

Aflatoxins are highly toxic secondary metabolies produced during the growth of several fungi, especiallyAspergillus flavus. AFB1 and AFB2 one of them which contaminates a wide variety of food and feed causing serioushealth problem when consumed by human or animals. This research was aimed to study the potency of Lactic AcidBacteria (LAB) in the inhibition of Aspergillus flavus growth and the production of Aflatoxin B2. Three species ofLAB i.e. Lactobacillus delbrueckii , L. fermentum, L. plantarum were investigated for their potential in inhibitingand degradation of Aflatoxin B2 as well as inhibiting fungal growth. The trial was designed into three variations ofeach isolate by challenging the fungal culture, before fungal inoculation, at the same time as fungal inoculation andafter fungal inoculation. It was found that all the three species of LAB are potential microorganism to inhibitfungal growth as indicated by the reduction of the dry weight of fungal mycelia compared with control.Quantification of Aflatoxin B2 showed that L. fermentum gave the strongest degradation of Aflatoxin B1 during 15days incubation, then followed by L. plantarum and L delbruekii with a reduction rate of 0,,2408 ppm, 0,3373 ppm,0,6393 ppm respectively, compared with control these are significantly different. These result conclude thatAflatoxin B2 can be degraded or prevented to be produced by A. flavus by applying LAB.
Penambahan Filtrat Tepung Umbi Dahlia (Dahlia variabilis Willd.) sebagai Prebiotik dalam Pembuatan Yoghurt Sinbiotik Alfaridhi, Khabib Khasan; Lunggani, Arina Tri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.421 KB) | DOI: 10.14710/bioma.15.2.64-72

Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus
Uji Kemampuan Produksi Fruktooligosakarida (FOS) dari Kelompok Aspergillus niger DUCC Whinarsih, Whinarsih; Lunggani, Arina Tri; Rukmi, MG Isworo
Bioma : Berkala Ilmiah Biologi Vol. 15, No.1, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.994 KB) | DOI: 10.14710/bioma.15.1.42-45

Abstract

Prebiotic is a food supplement that can not be digested in the human’s gut, it can stimulate the growth of beneficial microorganisms in the intestine and improve human’s health. FOS is a fructan type of oligosaccharide which is have a potential as a natural prebiotic, it  can be produced by several microorganisms, including A. niger group. The aim of this research was to examined the FOS production of   three isolates of A. niger group from DUCC collection (i.e. DUCC F123, DUCC F129 and DUCC F102). FOS production was determined by measuring the reducing sugar using  DNS method. The result showed that all isolates have the capability in producing  FOS suspected kestose with the degree of polymerization 3.545; 3.215; 3.049 respectively. Keywords:FOS, fructosyltransferase, Aspergillus niger, prebiotic
Optimasi Produksi Inulinase isolat P 12 pada Tepung Umbi Dahlia ( Dahlia variabilis Wild ) dengan Variasi Konsentrasi Nitrogen Organik dan Waktu Inkubasi Lunggani, Arina Tri; Wijanarka, Wijanarka; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 1, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.893 KB) | DOI: 10.14710/bioma.12.1.20-23

Abstract

Efforts to address health problems mainly related to the digestive tract is by consuming one prebiotic, eg fruktooligosakarida (FOS). FOS is a prebiotic that one species can be produced from the hydrolysis of inulin using inulinase enzyme. Isolate P12 is an isolate that has been proven to have high inulinase activity on standard medium inulin production. Inulinase production increase can be done by adding a source of organic nitrogen in the form of yeast extract in medium. The results indicate that the best on the concentration of nitrogen concentration P2 (Yeast extract 0.25%) with the activity of 0.7983 IU, while the best 12-hour incubation time with the activity of 0.7899 IU. Likewise for the best interaction P2 T2 treatment with inulinase activity of 0.9025 IU.
Isolasi dan Karakterisasi Morfologi Bakteri Halofilik dari Bledug Kuwu, Kabupaten Grobogan Sabdaningsih, Anindita; Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 22, No 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.106 KB) | DOI: 10.14710/bioma.22.1.46-52

Abstract

Bledug Kuwu is an area that has a fairly high salt content, this is caused by sea water trapped in the sedimentation process around the 18th century. Microorganisms that are able to live in extreme areas tend to have typical metabolic activities and can be utilized in industry. The purpose of this study was to characterize microbiologically, bacteria isolated from Bledug Kuwu, Kradenan District, Grobogan Regency, Central Java. The method used in this study was isolation on LB + NaCl media with NaCl levels of 10%, 12.5% and 15%. Characterization was done by Gram staining and motility test. The results obtained in samples from 3 sources, namely teak water, salt water and mud water, obtained 8 pure isolates. The seven isolates have the ability to grow optimally at 10% NaCl concentration so that these bacteria can be classified as moderate halophilic bacteria with the majority of species being coccus, Gram positive, and non motile.
Indigenous Trichoderma harzianum as Biocontrol toward Blight Late Disease and Biomodulator in Potato Plant Productivity Purwantisari, Susiana; Sitepu, Harum; Rukmi, Isworo; Lunggani, Arina Tri; Budihardjo, Kadarwati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26706

Abstract

Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.
Isolasi dan Karakterisasi Kapang Filoplan serta Serasah Daun di Lingkungan Laboratorium Biologi Universitas Diponegoro Dengan Metode Contact Plate Mahardika, Wahyu Aji; Dion, Romario; Qoys Naufal, Mochammad Fa’iq; Ramadhany, Warih; Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.6-10

Abstract

Fungi can be present on various substrates in the surrounding environment. The presence of these molds takes advantage of the nutrients that are attached to or contained in the substrate. The purpose of this study was to isolate and obtain various types of molds from various substrates. The method used in this study is a contact plate by attaching it to various objects or substrates, namely leaf litter, composter walls, tables, glass, and cabinets. The fungi obtained were 6 isolates, including those from the genus Aspergillus, Penicillium, Curvularia, and Alternaria,
Isolasi dan Potensi Enzim Hidrolase Bakteri Simbion Padina sp. dari Pantai Lengkuas Belitung Nur Jannah, Siti; Hanifa, Yumna Rahmadias; Utomo, Adi Budi; Dian Prambodo, Ashar Kurnia; Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.11-17

Abstract

Marine organism is one of the riches in the ocean of Indonesia. The benefits of sea use for new products produced are widely used and have high market demand. Enzymes that have marine interests have unique properties and have good benefits for industry. This study aims to isolate the bacteria that have symbionts with Padina sp and determine the potential of the enzyme hydrolase produced by these bacteria. Isolation is done by the spread plate method. Pure isolates obtained were then tested for the potential of the enzyme hydrolase on selective media. Clear zone measurements are performed to determine which bacterial isolates are good for enzyme production. The results obtained by 6 isolates of pure bacteria, all of which include Gram negative bacteria that form bacilli. All isolates had the ability to produce different Protease, Lipase, Amylase and Cellulase enzymes. The enzymes obtained from these symbiotic bacteria are expected to be used for industrial-scale production in Indonesia. In addition, the presence of this symbiont bacteria is able to reduce the level of exploitation of Padina sp and contribute to preserving the marine ecosystem.
Co-Authors Aninditia Sabdaningsih Anto Budiharjo Budi Raharjo Budihardjo, Kadarwati Cahyaningrum, Emi CATUR RIANI Choirunnisaa, Nadia Maharani Jasmine Dani Ika Purwaningsih Dewantoro, Giwang Dian Prambodo, Ashar Kurnia Diani Kurniawati Dion, Romario Dyah Wulandari Edwin Setiawan Eko Purnomo Endang Kusdiyantini Endang Kusdiyantini Enny Fachriyah Farikha, Siti Lutfiatul Hanifa, Yumna Rahmadias Hapzi Ali Hermin Pancasakti Kusumaningrum Khabib Khasan Alfaridhi, Khabib Khasan Khodijah Baharun Mahardhika, Wahyu Aji Mahardika, Wahyu Aji MG Isworo Rukmi Muhammad Amal Nurhakim, Muhammad Amal Nur Jannah, Siti Purwantisari, Susianna Qoys Naufal, Mochammad Fa’iq Raden Mohamad Herdian Bhakti Ramadhany, Warih Sari, Rachel Dewita Sitepu, Harum Siti Nur Jannah Soni Nugraha Anwar Sri Pujiyanto Susiana Purwantisari Syawal Nurangga Kodhatin Utomo, Adi Budi Whinarsih, Whinarsih Wijanarka Wijanarka Wijanarka, W