High Resolution Microsatellite Marker Analysis of Some Rice Landraces Using Metaphor Agarose Gel Electrophoresis

K. Kristamtini (Assesment Institute of Agriculture Technology, Yogyakarta, Indonesia)
T. Taryono (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Panjisakti Basunanda (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Rudi Hari Murti (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia)

Indonesian Journal of Biotechnology
Vol 20, No 1 (2015)

Microsatellite markers or simple sequences repeats are DNA - based molecular techniques that areused to see the different among accessions and inbred lines. There are three methods to analysis the results ofthe polymerase chain reaction of microsatellite markers namely polyacrylamide gel electrophoresis (PAGE),capillary electroforesis, and Metaphor Agarose Gel Electroforesis (MAGE), and the Use of MAGE assessedmore easily and economically the polymorphic pattern of DNA markers. This study aimed to obtain fast,effective and efficient in term of easy and cheap technique to identify microsatellite markers of some blackrice cultivars and F2 populations from crosses between black with white rice. The results showed that MAGEsuccessfully separated clearly SSRs alleles with different sizes of less than 25 bp .