Indonesian Journal of Biotechnology
Vol 17, No 1 (2012)

Regression Analysis for the Identification of RAPD Markers Linked To Drought Tolerance in Sorghum

Paramita Cahyaningrum (Faculty of Science and Mathematics, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia)
T. Taryono (Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Anto Rimbawanto (Center for Forest Biotechnology Research, Ministry of Forestry, Yogyakarta, Indonesia)



Article Info

Publish Date
03 Jun 2012

Abstract

Sorghum (Sorghum bicolor) can actually withstand in dry or drought condition better than other crops,therefore it can be grown at different agroclimatic conditions and its product can be used for different purposessuch as food, feed and industrial raw material. However at severe condition, the productivity will also dropdrastically. The aim of this research was to identify RAPD marker linked to the drought tolerance. In thisresearch, varieties of sorghum used as research materials were Durra, Zhengzu, the mutants of Durra andZhengzu (from 300 Gy gamma radiation) B-100 and Zh-30, and the F2 seeds from Zh-30 x B-100 and B-100 xZh-30. Drought screening was carried out using 0.3 % KI during sorghum vegetative stage. DNA extractionwas done using a modified CTAB method. PCR was carried out for RAPD analysis. PCR amplification productswere scored and analyzed using SAS program. The result showed that potassium iodide can be used fordrought screening during the vegetative stage and regression analysis using the logistic method can be usedto identify RAPD markers that is linked to drought tolerance in sorghum. The logistic analysis showed thatband A8-480 was linked to drought tolerance in sorghum.

Copyrights © 2012






Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...