3BIO: Journal of Biological Science, Technology and Management
Vol. 8 No. 2 (2026)

Effect of 6-Benzylaminopurine (BAP) on Apical Shoot Growth of Ramie (Boehmeria nivea L. Gaud.) Wonosobo Clone In Vitro

Nuraini, Anne (Unknown)
Hutajulu, Esther Christy Natasya (Unknown)
Wulandari, Asri Peni (Unknown)
Elisabeth, Pauline (Unknown)
Muhammadiyah, Muhammadiyah (Unknown)



Article Info

Publish Date
23 Apr 2026

Abstract

Ramie (Boehmeria nivea L. Gaud.) is considered an important natural fiber crop, particularly due to its profitability. However, its propagation still faces some ultrapractical cases. When typical vegetative styles are used, the number of new shops created is relatively low, which makes large-scale production less effective. To address this conclusion, an in vitro study was conducted on the initial Wonosobo ramie clone. The aim was to determine how different concentrations of 6- benzylaminopurine (BAP) affect apical shoot conformation. Six concentrations of BAP (0, 0.5, 1.0, 1.5, 2.0, and 2.5 ppm) were examined in a completely randomized design with four replications. During the cultivation period, several responses were recorded. The study observed the time for shoot emergence, number of shoots formed, and leaf development across all treatments. The response varied across different concentrations, with certain treatments exhibiting slower shoot formation and lower quantities. The highest shoot accumulation was obtained at 1.5 ppm BAP. These results indicate that BAP is important for stimulating shoot extension, but its concentration must be carefully optimized, as both too low and too high levels are less effective. Based on these findings, BAP with 1.5 ppm is optimal for enhancing in vitro shoot production of ramie.

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Journal Info

Abbrev

3bio

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Neuroscience

Description

3Bio: Journal of Biological Science, Technology and Management is an interdisciplinary peer-reviewed journal in a wide aspect related to the field of life sciences and other related fields of study. The journal aims to promote scientific discourse and disseminate research on various branches and ...