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All Journal 3BIO: Journal of Biological Science, Technology and Management Berita Biologi
Elisabeth, Pauline
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Neuroscience

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Effect of 6-Benzylaminopurine (BAP) on Apical Shoot Growth of Ramie (Boehmeria nivea L. Gaud.) Wonosobo Clone In Vitro Nuraini, Anne; Hutajulu, Esther Christy Natasya; Wulandari, Asri Peni; Elisabeth, Pauline; Muhammadiyah, Muhammadiyah
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 2 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.2.2

Abstract

Ramie (Boehmeria nivea L. Gaud.) is considered an important natural fiber crop, particularly due to its profitability. However, its propagation still faces some ultrapractical cases. When typical vegetative styles are used, the number of new shops created is relatively low, which makes large-scale production less effective. To address this conclusion, an in vitro study was conducted on the initial Wonosobo ramie clone. The aim was to determine how different concentrations of 6- benzylaminopurine (BAP) affect apical shoot conformation. Six concentrations of BAP (0, 0.5, 1.0, 1.5, 2.0, and 2.5 ppm) were examined in a completely randomized design with four replications. During the cultivation period, several responses were recorded. The study observed the time for shoot emergence, number of shoots formed, and leaf development across all treatments. The response varied across different concentrations, with certain treatments exhibiting slower shoot formation and lower quantities. The highest shoot accumulation was obtained at 1.5 ppm BAP. These results indicate that BAP is important for stimulating shoot extension, but its concentration must be carefully optimized, as both too low and too high levels are less effective. Based on these findings, BAP with 1.5 ppm is optimal for enhancing in vitro shoot production of ramie.
INDUCTION OF SHOOT FORMATION IN STRAWBERRY (Fragaria × Ananassa DUCH.) CLONE BAT 1 IN VITRO USING VARIOUS TYPES AND CONCENTRATIONS OF CYTOKININS Elisabeth, Pauline; Anne Nuraini; Murgayanti; Muhammadiyah
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.11373

Abstract

Strawberry (Fragaria × ananassa Duch.) is a high-value subtropical horticultural crop. One promising local variety under development is clone BAT 1. To accelerate propagation, tissue culture techniques are needed, especially during bud initiation, which is influenced by cytokinin type and concentration. This study used a Completely Randomized Design (CRD) with 7 treatments and 4 replications, using Murashige and Skoog (MS) medium supplemented with Thidiazuron (TDZ) and 2-Isopentyl adenine (2-iP) at concentrations of 0; 0.50; 0.75; 1 ppm TDZ and 1; 2; 3 ppm 2-iP. The procedures included explant preparation, sterilization of equipment and area, stock solution preparation, media formulation and sterilization, explant sterilization, and planting into treatment media. Results showed that cytokinins at 0.50–1 ppm significantly enhanced bud number, leaf number, culture height, and fresh weight.  The most effective treatments were 1 ppm 2-iP (5.24 buds; 5.90 leaves; 2.23 cm height) and 0.50 ppm TDZ (1.36 g fresh weight), indicating their potential for improving in-vitro propagation of clone BAT 1.
Co-Authors Anne Nuraini Asri Peni Wulandari Hutajulu, Esther Christy Natasya Muhammadiyah Muhammadiyah, Muhammadiyah Murgayanti