Molecular Identifcation of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product

Helen Joan Lawalata (Departement of Biology, Faculty of Mathematics and Natural Science, Manado State University, Manado, Indonesia)
Langkah Sembiring (Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia)
Endang Sutriswati Rahayu (Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia)

Indonesian Journal of Biotechnology
Vol 16, No 2 (2011)

Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditionalfermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilagebacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. Oneisolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilagebacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producingantimicrobial agents from bakasang were identifed as Pediococcus acidilactici.