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All Journal Jurnal Bios Logos JSME MIPA UNIMA Jurnal Produksi Tanaman Indonesian Journal of Biotechnology Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus SOSCIED Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Makara Journal of Science Indonesian Biodiversity Journal Rumphius Pattimura Biological Journal Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika Jurnal Pengabdian Masyarakat dan Riset Pendidikan Indonesian Biodiversity Journal Polygon: Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Algoritma: Jurnal Matematika, Ilmu Pengetahuan Alam, Kebumian dan Angkasa J-CEKI Konstanta : Jurnal Matematika dan Ilmu Pengelatuan Alam
Helen Joan Lawalata
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product Lawalata, Helen Joan; Sembiring, Langkah; Rahayu, Endang Sutriswati
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

AbstractTwenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional fermented fish product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage bacteria. This strain was further identified by 16S rRNA gen sequence comparison. Isolates LAB producing antimicrobial agents from bakasang were identified as Pediococcus acidilactici.Keywords : Bakasang, LAB, antimicrobial, phenotypic characteristics, 16S rRNA gene
Isolasi dan Identifikasi Bakteri Asam Laktat dari Fermentasi Anggur Buah Pala (Myristica fragrans Houtt) Sebagai Penghasil Eksopolisakarida Lawalata, Helen Joane; Rompas, Christny Ferdina; Kansile, Elma Fridayanti
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 1 (2020): JANUARI-FEBRUARI
Publisher : Universitas Negeri Manado

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. Nutmeg fruit is widely used in the medicine formula and also various kinds of processed foods and beverages. Nutmeg meat can be processed into syrup, fruit juice, jam, dodol pala and wine. The fruit is believed to be one of the natural habitats of bacteria, including lactic acid bacteria (BAL). The study aims to determine the type of BALES isolated in nutmeg grapes (Myristica fragrans Houtt) which has the ability to produce exopolysaccharides. Based on the results of the study obtained 15 BAL isolates namely AP1, AP2, AP3, AP4, AP5, AP6, AP7, AP8, AP9, AP10, AP11, AP12, AP13, AP14, and AP15. The exopolysaccharides produced by the isolates are 400 mg/L, 530 mg/L, 1200 mg/L, 730 mg/L, 60 mg/L, 460 mg/L, 600 mg/L, 200 mg/L, 930 mg/L, 1130 mg/L, 200 mg/L, 130 mg/L, 1000 mg/L, 800 mg/L. The next 15 isolates are identified using the profile matching method. The results of the identification show that 15 BAL isolates were included in the genus Lactobacillus and are a species of Lactobacillus bulgaricus.    Keyword : Isolation, Identification, nugmeg grapes (myristica fragrans houtt), lactic acid bacteria, exopolysaccharides
PEMBELAJARAN BIOLOGI MELALUI PRAKTIKUM UJI GOLONGAN DARAH BERBASIS DISCOVERY LEARNING UNTUK MENINGKATKAN HASIL BELAJAR SISWA SMP NEGERI 3 TONDANO Muli, Efel J. R.; Sumampouw, Herry M.; Lawalata, Helen J.
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 1 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Masalah yang melatar belakangi penelitian ini setelah observasi awal dilakukan yaitu metode pembelajaran masih bersifat konvensional dan praktikum penggolongan darah kurang dilakukan sehingga pemahaman terhadap konsep biologi sulit dicerna. Tujuan penelitian ini untuk meningkatkan hasil belajar pada pembelajaran biologi melalui praktikum uji golongan darah berbasis discovery learning untuk meningkatkan hasil belajar siswa SMP Negeri 3 Tondano. Penelitian ini menggunakan metode penelitian tindakan kelas yang terdiri dari 4 tahap yaitu: a. Perencanaan, b. Pelaksanaan, c. Observasi, dan d. Refleksi. Subjek penelitian ini adalah siswa kelas VIII b dengan jumlah siswa 20 orang siswa yang terdiri dari 12 siswa laki-laki dan 8 siswa perempuan. Penelitian ini dilakukan dalam dua siklus. Siklus pertama dengan materi sistem peredaran darah, jumlah siswa yang memenuhi KKM 12 orang atau 60% secara klasikal dan pada siklus dua dengan materi yang sama sistem peredaran darah, jumlah siswa yang memenuhi nilai KKM 75 yaitu 18 orang atau 90% secara klasikal dan tidak tuntas 2 siswa atau 10 % diadakan remedial. Dengan demikian pembelajaran melalui praktikum uji golongan darah berbasis discovery learning dapat meningkatkan hasil belajar siswa. Kata kunci: Discovery learning, hasil belajar, uji golongan darah
ANALISIS KANDUNGAN BAKTERI ESCHERICHIA COLI PADA AIR SUMUR GALI DI KECAMATAN LIRUNG KABUPATEN KEPULAUAN TALAUD Lawalata, Helen Joan; Tengker, Anita C. Ch.; Maradesa, Sartika
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 2 (2020): MARET - APRIL
Publisher : Universitas Negeri Manado

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ABSTRACT. Research has been carried out to determine the content of the bacterium escherichia coli in well water dug in the sub-district of Lirung, regency of the Talaud Islands. Water is one of the sources of living needs of living things, humans, animals and plants. Well water is a means of clean water from the soil for daily use, such as drinking water, cooking, washing and bathing. Therefore, the water must be pollution-free and meet the quality requirements for clean water. e.coli bacteria are bacteria that generally live in the intestines of humans and animals. This research uses a quantitative descriptive method. The results showed that 9 samples of water wells taken from 3 sites in the Lirung sub-district, in the regency of the Talaud Islands, contained e.coli bacteria which exceeded the maximum threshold not in accordance with the regulations of the Ministry of Health RI No.  416 / MENKES / PER / IX / 1990   Keywords: Bacteria Escherichia coli, Dug Well Water.
Molecular Identifcation of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product Helen Joan Lawalata; Langkah Sembiring; Endang Sutriswati Rahayu
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.001 KB) | DOI: 10.22146/ijbiotech.16368

Abstract

Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditionalfermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilagebacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. Oneisolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilagebacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producingantimicrobial agents from bakasang were identifed as Pediococcus acidilactici.
ANALYSIS OF THE EFFECT OF PGPR FERTILIZER USE ON GROWTH ACCELERATION OF CHERRY TOMATO (Solanum lycopersicum var. cerasiforme) Genade Kojongian; Orbanus Naharia; Helen Lawalata
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Abstract

Cherry tomatoes are a type or commodity of horticulture that has a very relatively high economic value compared to ordinary tomatoes. Cherry tomato plants (Solanum lycopersicum var,. cerasiforme. It is one of the local plants that is neglected and not used and is often considered as a wild plant. This study aims to determine the effect of PGPR fertilizer application on the growth of cherry tomato plants. This research was carried out in the urban village). Rerewokan, West Tondano sub-district starting from August to November 2021. The method used is a quantitative method with the type of experimental research in the form of a Randomized Block Design (RAK) with five types of treatment, each repeated four times. P1 = without using fertilizer, P2 = 100ml, P3 = 200ml, P4 = 300ml, P5 = 400ml, so there were 20 treatment combinations, and the data obtained were then analyzed using analysis of variance (ANOVA). From the results of this study, it showed that good plant height growth was found in treatment P4 with the application of a mixture of PGPR fertilizer concentration of 300ml / 2 liters of water, because there was the most effective growth, while the growth in the number of leaves and the number of fruits was very good in treatment P2 with the addition of a mixture. PGPR fertilizer concentration 100ml / 2 liters of water.
Isolation, Identification and Antibacterial Testing, Indigenous Bacteria from Apis Mellifera's Honeycomb North Sumatra Origin Esibrena Br Kemit; Yermia S. Mokosuli; Helen J Lawalata
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.4343

Abstract

Honeycomb can be used as a source of antibacterial, this is due to the content of secondary metabolites in the form of flavonoids in the honeycomb which serves as a protective determinant of honey quality. This study aims to isolate, identify and test antibacterial isolates of Indigenous bacteria from Apis mellifera nests against gram-positive bacteria S. aureus and gram-negative bacteria E. coli. This research used descriptive method and the research data were obtained through laboratory experiments. The results of the isolation stage of indigenous bacteria obtained seven (7) isolates AM1, AM2, AM3, AM4, AM5, AM6, AM7 Indigenous bacteria from Apis mellifera bee hives. AM1 and AM4 isolates were similar to the Enterobacter genus, AM2 isolates to Corynebacterium genus, AM3 and AM6 isolates to Paracoccus genus, and AM5 and AM7 isolates to Azotobacter Sp. The seven isolates of Indigenous bacteria have potential as antibacterial and the diameter of the inhibition zone against E. coli bacteria is AM1 (8.88 mm), AM2 (8.65 mm), AM3 (8.03 mm), AM4 (6.41 mm) , AM5 (9.07 mm), AM6 (9.53 mm) and AM7 (9.44 mm) while against S. aureus AM1 (9.08 mm), AM2 (9.23 mm), AM3 (9.15 mm), AM4 (8.70 mm), AM5 (10.44 mm), AM6 (11.56 mm) and AM7 (9.0 mm).
STUDY OF MANGROVE VEGETATION COMMUNITY AT BUNAKEN NATIONAL PARK AREA, TELING VILLAGE Febiola Gabriela Tular; Helen J Lawalata; Marthy L. S. Taulu
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

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Mangrove vegetation generally grows to form a zoning starting from a beach to a view of meters inland. Mangrove zoning consists of Avicennia plant species that face directly toward the sea and behind or on the edge of the Avicennia plant. There is the Riophora plant. Towards the mainland, there are Bruguiera plants and, finally, the Ceriops plant which grows and interacts with small bushes. Each type of Mangrove plant has a level; the first is the seedling level, the second is the sapling level, the third is the pole level, and the fourth is the tree level. This study aims to analyze the Mangrove Vegetation Community in Bunaken Nation Park, Teling Village, using a descriptive survey method and constructing three transects, which are transected measuring 10×10 m2, 5×5 m2, and 2×2 m2. The result on each transect shows that in Bunaken National Park, Teling Village, there were types of Mangrove Vegetation Communities, namely Avicennia plant species with a total of 129, Rhizophora plant species with a capacity of 122 and Bruguiera plant species with a total of 91, where each plant type it has levels with different numbers.
ANALISIS KANDUNGAN GIZI DAN UJI BAKTERI COLIFORM PADA TEMPE BIJI BUAH PANGI Pangium edule Reinw Maiko Polandos; Mercy Rampengan; Helen J. Lawalata; Anita C.C. Tengker; Verawati I.Y. Roring
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 8 No. 2 (2023): Bioma : Juli - Desember 2023
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Pangi (Pangiun edule Reinw) merupakan tanaman hutan dan banyak tersebar di wilayah Indonesia. Kelimpahan tanaman pangi belum sejalan dengan pengoptimalam dan pemanfaatannya disebabkan kurangnya informasi akan manfaat cara pengolahan bahan pangan. Penelitian ini dilakukan dengan memanfaatkan biji buah pangi sebagai bahan dasar pembuatan tempe. Tujuan penelitian ini untuk melakukan penelitian mengenai kandungan gizi dan uji bakteri yang terdapat pada tempe biji pangi. Analisis data dilakukan dengan metode deskriptif kuantitatif. Hasil penelitian menunjukkan nilai kadar air 64,96%, kadar abu 0,64%, kadar protein 11,61%, kadar lemak 16,97%, kadar serat 7,20%, kadar karbohidrat 5,82%, dan uji cemaran bakteri menunjukkan nilai 93 APM/g.
IDENTIFICATION OF THE TYPE OF FERNS IN THE FOREST AREA OF NOONGAN VILLAGE YOSUA LINU; Helen J Lawalata; Rievo Djarang
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

Ferns (Pteridophyta) are spore-forming cormophytes that can live in a variety of habitats both as epiphytes, terrestrial and aquatic. In the forest area of Noongan Village, many potentials play an important role in developing forest ecosystems. One such potential is ferns. The results of the study were eight types of ferns in the forest area of Noongan village which were divided into two plots of 50x50 meters at different heights, namely plot I at an altitude of 610 masl with a total of 380 ferns with the percentage: Paku Garuda (Pteridium aquilinium) 0.20 %, Elephant fern (Angiopetris avecta Hoofm) 0.01%, Red rane fern (Selaginella australis) 0.01%, Paku Kikir (Stenosomia Sp.) 0.14%, Ferret fern (Nephrolepis biserrataSchott.) 0, 21%, cecerenean fern (Nephrolepis falcata) 0.16%, suplir fern (Adiantum.) 0.12%, andam fern (Dicranopetris linearis) 0.12%. And plot II at an altitude of 869 meters above sea level with a total of 179 ferns with a percentage of: Garuda fern (Pteridium aquilinium) 0.21%, elephant fern (Angiopetris avectaHoofm) 0.01%, miserly fern (Stenosomia Sp.) 0, 17%, Paku harupat (Nephrolepis biserrate Schott.) 0.21%, Paku cecerenean (Nephrolepis falcata) 0.15%, Suplir (Adiantum.) 0.10%, Paku andam (Dicranopetris linearis) 0.12%. In the plot I, the types and number of ferns were higher than the types and numbers of ferns in plot II. No red fern (Selaginella australis) was found in plot II. And the plant species Paku Harupat (Nephrolepis biserrata Schott). The red fern has the highest number, and the red fern (Selaginella australis.) has the least amount. The difference in altitude greatly affects the surrounding environment. So that these differences greatly affect the number of types and numbers of ferns
Co-Authors Akay, Fanesa Anita Costanci Christine Tengker Aser Yalindua Bricita Regina Warangkiran Christny Ferdina Evie Rompas Daud, Putri Debby Jacqualine Jochebed Rayer Decky D. W. Kamagi Delvy Regina Leunufna Elsa Aru Emma M Moko Endang Sutriswati Rahayu Esibrena Br Kemit Etin Ruban Febiola Gabriela Tular Fernando Andre Watung Futwembun, Fransiska Genade Kojongian Helena, Maria Helma Fitriani Herry Maurits Sumampouw Hotni Doani Purba IRIANI SETYAWATI Iriani Setyawati Jantje Ngangi Jefry Jack Mamangkey Jelita Pricilya Tompolumiu Kansile, Elma Fridayanti Kembuan, Patricia Pericilia Langkah Sembiring LANGKAH SEMBIRING Letsoin, Imanuel Junisa Adolfina Lidya Yangse Lumowa Livana Dethris Rawung Luis Sandi Najoan Maiko Polandos Manampiring, Nonny Maradesa, Sartika Marchio Yosua Gratia Mamesah Mariana Rengkuan Marsanda Tandiapa Marthy L. S. Taulu Meity Nelltje Tanor Melany Piri Meyke Paat Miracle Abbighael Varencia Welan Moko, Emma Mokoginta, Ayatola R. Mokosuli Yermia Samuel Mokosuli, Yeremia Samuel Mulder, Keysia Stephanie Tesalonica Muli, Efel J. R. Nangoy, Wiesye M. S> Nonny Manampiring Nova L.I.M Ogi Nuriyati Hadia Orbanus Naharia Orbanus Naharia Pelealu, Irna W. Posumah, Danny Posumah, Danny Christian Rahardiyan, Dino Rahayu, Endang Sutriswati Rampengan, Mercy Maggy Franky Regina R. Butarbutar Revolson Mege Rey, Feyne Jelita Rievo Djarang Rompas, Christny Ferdina Evie Roring, Verawati I. Y Roring, Verawati I. Y. Runtunuwu, Yudistira Deyvan Salaila, Tania Jennet Sambeka, Delvino Sambuaga, Natalia V. Samuel, Mokosuli Y. Simbolon, Kristina Br Sisilia Domitila Sangur Tanorl, Meity N. Tatebale, Rivaldo Tedang, Bybyani Tuelah, Syela Nathasya Utari Satiman Verawati I Y Roring Verawati I.Y. Roring Verawati I.Y. Roring Verawati Ida Yani Roring Watugigir, Gerfindo Y. G Wiesye Nangoy Yeremia S. Mokosuli YOSUA LINU Zusje W. M. Warouw