Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

Eny Rahma Wati (Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University)
Sasangka Prasetyawan (Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University)
Chanif Mahdi (Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University)
Arie Srihardyastutie (Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University)
Made Oka Adnyana (Department of Medicine, Faculty of Medicine, Udayana University)
Aulanni’am Aulanni’am (Department of Biochemistry, Faculty of Veterinary Medicine, Brawijaya University)

The Journal of Pure and Applied Chemistry Research
Vol 7, No 1 (2018): Edition January-April 2018

This research studies the effect of anthocyanin from purple sweet potato in the cerebellum of stroke ischemic rats by MCAO (Middle Cerebral Artery Occlusion) technique. This technique was carried out by ligating the blood flow in ECA and CCA region for 3 hours, followed by reperfusion. The MCAO technique proved as a technique for preparing ischemic stroke rats. This technique induces releasing of BDNF and VEGF in the cerebellum of ischemic stroke rats. The level of BDNF measured by ELISA technique and VEGF expression used immunohistochemistry technique. The results showed that anthocyanin from purple sweet potato increased the level of both BDNF and VEGF expression in the cerebellum in ischemic stroke rats. It is suggested that anthocyanin could be used as a therapeutic agent for ischemic stroke.