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All Journal HAYATI Journal of Biosciences VALENSI JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmu dan Teknologi Hasil Ternak The Journal of Experimental Life Sciences (JELS) The Indonesian Green Technology Journal Natural B The Journal of Pure and Applied Chemistry Research Journal of Mathematical and Fundamental Sciences JURNAL PENGABDIAN KEPADA MASYARAKAT Science and Technology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry JMM (Jurnal Masyarakat Mandiri) JKPK (Jurnal Kimia dan Pendidikan Kimia)

Arie Srihardyastutie

Chemistry Department Brawijaya University

Author-ID : 2844125

Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Other

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Bioaccumulation of Heavy Metals Pb, Cd And Zn on Bentos in the Estuary of Porong River Sidoarjo Yudita Prihatini Puji Rahma Sari; Barlah Rumhayati; Arie Srihardyastutie
Natural B, Journal of Health and Environmental Sciences Vol 4, No 1 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

This research is done to know the influence of sort and size of biota, pH dan Salinity of water body toward Bioconcentration Factor (BFC) heavy metal, and protein characterization in biota contaminated with heavy metal. Water sampling, biota and sediment are done in two locations in estuary River Porong i.e. estuary watery nearby river and estuary nearby sea. The parameter of water circumstance employed is pH and salinity. The analysis of metal in water sample and biota is done after destruction uses aqua regia. The fraction of metal which is easily switched in metal sediment is analyzed after extraction uses acetate acid. The concentration of metal is analyzed with spectrophotometer of atom absorptivity. Characterization of protein in biota sample is done through electrophoresis employing SDS-PAGE. The result of this research is seen that Sergestide(shrimp) and Corbulidae(shellfish) possess the same big ability in accumulating metal Pb, Cd and Zn. Biota with big size (>1cm) has BCF higher than biota with small size(<1cm). pH and salinity of water body does not influence BCF value. System of biota defense contaminated with heavy metal is marked by protein hsp40(35 kDa), hsp60(50kDa) and hsp90(90kDa).

Hexavalent Chromium Removal from Electroplating Waste Using Biochitin from L.Vanamme Shrimp Shell Waste Angelina Rosmawati; Barlah Rumhayati; Arie Srihardyastutie
The Journal of Pure and Applied Chemistry Research Vol 9, No 2 (2020): Edition May-August 2020
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2020.009.02.504

The increasing number of electroplating industries in this globalization era has a negative impact on the environment because of its liquid waste containing high concentrations of hexavalent chromium, Cr (VI). To overcome this problem, biochitin extracted from white shrimp shell waste (L.vanamme) has been used to adsorb Cr (VI) from electroplating waste. Biochitins were extracted through single and sequential fermentation method using L.plantarum and B.thuringiensis bacteria. The optimization process of Cr (VI) removal was done by using biochitin from different methods, at various mass of biochitin, the time of adsorption, and Cr (VI) concentration. Results showed that biochitin produced from the sequential fermentation method could optimally adsorb Cr (VI). By using 2 g of biochitin, Cr (VI) could be removed as much as 51.99 ± 0.41% during 6 hours. At the optimum condition, the selected adsorbent could remove Cr (VI) in the concentration range of 9.38 to 92.39 mg/L, which showed the adsorption capacity of 0.915 mg/g. The capability of biochitin for Cr (VI) removal was reinforced by the SEM EDS images, which show the presence of chromium compounds in the biochitin.

Study of Antocyanins Activity from Purple Sweet Potato for Reducing Apoptotic Cells Expression of The Cerebellum On Ischemic Stroke Rats Latifah Tribuana Dewi; Made Oka Adnyana; Chanif Mahdi; Sasangka Prasetyawan; Arie Srihardyastutie; Aulanni’am Aulanni’am
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

This study aims to determine anthocyanin antioxidant performance with a dose of 2 cc/day based on the expression of apoptotic cells on ischemic stroke rats cerebellum. Research was conducted using ischemic stroke rats (Rattus norvegicus) that prepared by ligated for 3 h on the Common Carotid Artery (CCA) and External Carotid Artery (ECA) followed by reperfusion that commonly known as MCAO (Middle Cerebral Artery Occlusion). The anthocyanin extract was characterized by LC-MS and its IC50 was measured by DPPH method. The rats were divided into five groups 1) negative control; 2) reperfusion 1 h; 3) reperfusion 72 h; 4) reperfusion 24 h, with anthocyanin therapy; 5) reperfusion 72 h, with anthocyanin therapy. The results of LC-MS showed that anthocyanin from purple sweet potato extracts contained Petunidin-3,5-O-diglucoside (Pt-DG) with an IC50 value of 22.16 μg/mL, categorized as very strong antioxidant. The results showed that apoptotic cells expression of cerebellum decreased significantly (p<0.01) after 72 h reperfusion with anthocyanins therapy until 2.42%. The current work proved that anthocyanin extract effectively suppresses the apoptotic cell’s expression of the cerebellum on stroke ischemic rats.

Optimization Of Elevating Blood Uric Acid Levels With High Purine Diet Fitria Rahmawati; Putranty Widha Nugraheni; Chanif Mahdi; Arie Srihardyastutie; Sasangka Prasetyawan
The Journal of Pure and Applied Chemistry Research Vol 7, No 1 (2018): Edition January-April 2018
Publisher : Chemistry Department, The University of Brawijaya

Exploration of the use of medicinal plants to lower uric acid levels has been widely practiced. Stages of new drug development research is a preclinical test using experimental animals, therefore the manufacture of an animal model of hyperuricemia is necessary. This study aims to determine the optimal induction of uric acid to increase blood uric acid levels by administering high purine foods such as cow’s liver, cow’s spleen, Gnetum gnemon, emping and fried peanuts. Eighty male white rats were used individuals to be divided into 4 groups, they were: (I) cow’s liver, (II) cow’s liver and cow’s spleen, (III) cow’s liver, cow’s spleen and boiled gnetum gnemon beans, and (IV) cow’s liver, cow’s spleen, emping and fried peanuts. This study using easy touch GCU to measure blood uric acid level. The result of statistical analysis of uric acid level means with 5 times repetition using One Way ANOVA showed that there was a very significant difference between treatments (p <0,01). The results concluded that high purine diet in group I, II and III had not been able to increase uric acid levels significantly. High purine diet group IV was able to increase blood uric acid levels significantly to make the rats experiencing hyperuricemia with the level of 6.54 mg/dL on day 7 and 13.79 mg/dL on day 14.

Antinutritional Content, Flour Characteristics, and Protein Profiles of Taro Tubers (Colocasia esculenta) by Fermentation with Lactobacillus plantarum Maria Natalia Wewo; Sasangka Prasetyawan; Arie Srihardyastutie
The Journal of Pure and Applied Chemistry Research Vol 7, No 3 (2018): Edition September-December 2018
Publisher : Chemistry Department, The University of Brawijaya

The purpose of this research was studying the optimum condition, the change of oxalate content, the characteristic of flour, and protein profile of taro tuber that fermented with Lactobacillus plantarum. The optimum condition of fermentation was determined based on the lowest oxalate content in flour. The optimum conditions of fermentation studied were variation of pH (4-6), temperature (30⁰–50 ⁰C) and incubation time (6–48 hours). The result showed that the optimum condition of fermentation was achieved at pH 5, 35 °C in 48 h incubation time. The fermented taro flour characteristics were the increasing level of starch (4.76%), amylose (0.43%). amylopectin (4.36%) and protein (0.99%). The fermented taro protein profiles showed that the allergenic proteins were not present, suggesting those proteins were missing due to fermentation process.

Antibacterial Activity and Phytochemical Analysis of Edible Seaweed Eucheuma spinosum Against Staphyloccocus aureus Anna Safitri; Arie Srihardyastutie; Anna Roosdiana; Sutrisno Sutrisno
The Journal of Pure and Applied Chemistry Research Vol 7, No 3 (2018): Edition September-December 2018
Publisher : Chemistry Department, The University of Brawijaya

The current work aims to determine antibacterial activities of ethanol extracts of Eucheuma spinosum against Staphylococcus aureus. These were showed through inhibition zones formation, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) values. The MIC was determined using dilution test tube method, and the MBC was calculated using streaking method. Qualitative test was applied to determine phytochemical compounds in ethanol extract of E. spinosum. Results showed that ethanol extracts of E. spinosum at concentrations of 1%; 2%; 5%, and 10% (v/v) resulted in inhibition zones of 1.98; 4.14; 7.42; and 10.27 mm, respectively. In addition, concentration of 10% (v/v) was the MIC, and 15% (v/v) was the MBC values of E. spinosum extracts against S. aureus. The phytochemical analysis showed that alkaloids and saponins were the main components detected. Alkaloids positive tests were observed by the formation of brown sediment with Wagner’s reagent, yellow sediment with Mayer’s reagent, and orange sediment with Dragendorff’s reagent. Saponins positive results were detected by the formation of stable foam when the extracts reacted with water and HCl. Overall, these results suggested that E. spinosum extracts have antibacterial properties against Gram-positive bacteria, i.e. S. aureus.

Lactobacillus plantarum Fermentation Effect on Tannin Reduction, Proximate Analysis, and Protein Profiles of Ganyong (Canna edulis Kerr) Flour Azor Yulianus Tefa; Arie Srihardyastutie; Sasangka Prasetyawan
The Journal of Pure and Applied Chemistry Research Vol 8, No 1 (2019): Edition January-April 2019
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2019.008.01.437

One of the problems in using ganyong (Canna edulis Kerr)as food is the presence of tannin, an antinutritional substance, that can reduce the nutritional quality of ganyong. The purpose of this study was aimed to analyze the effect of fermenting ganyong using Lactobacillus Plantarumbacteria on the chemical compounds and the reduction of tannin level in its flour. Theresults showed that the optimum conditions of fermenting ganyong were at pH 6, the temperature of 40 oC, and at the fermentation time of 36 hours. At the optimum conditions, Lactobacillus Plantarumwas able to decrease tannin content in ganyong from 2.53 mg/mL to 0.84 mg/mL. The reduction of tannin content is due to the activity of tannase produced by the microorganisms. Fermentation caused the enrichment protein content, from 1.87% to 2.01% and the reduction of starch, amylose, and amylopectin contents to 51%, 16.82%, and 38.08% from 60.19%, 18.27%, and 43.49% respectively. SDS-PAGE results showed that the protein profiles changed in ganyong sample after the fermentation process. Protein with a molecular weight of 72.49 kDa which showed in the non-fermented ganyong, did not appear in the fermented ganyong, and protein band intensities also showed changes.

Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus) Faijal Fachrudin Mbabho; Aulanni’am Aulanni’am; Arie Srihardyastutie
The Journal of Pure and Applied Chemistry Research Vol 7, No 3 (2018): Edition September-December 2018
Publisher : Chemistry Department, The University of Brawijaya

Essential oils of canarium tree resin (Canarium indicum L) have anti-inflammatory and antioxidant activity. Inflammation of the bronchi and airway remodeling is a major factor in the occurrence of asthma. The purpose of this study was to study the potential of essential oils of canarium tree resin on the expression of TNF-α, changes in bronchial epithelial cell features, MDA levels and protease activity from OVA and LPS exposure in a rat model of asthma. This study used four groups of rat, the negative control group, asthma group, therapy group dose 25 mg/kg BB and 50 mg/kg BB. TNF-α expression was calculated using Walter calculations, histopathologic observation of bronchi was observed using Olympus BX51 microscope, MDA level was measured using Thiobarbituric Acid (TBA) method and measurement of protease activity was done by spectrophotometric method, and analyzed by ANOVA. The results showed that the therapy of essential oils of canarium tree resin at 25 mg/kg BB and 50 mg/kg BB significantly (P<0.05), after treatment for seven days decreased, MDA 44%, Protease activity 46%, improved bronchial epithelium and TNF-α expression by 51%. This study proves that essential oils of canarium tree resin can be effectively used as therapy in asthma model rats.

Effects of Ruellia tuberosa L. Root Extract on the Pancreatics of Diabetic Rat Muhammad Asadullah; Arie Srihardyastutie; Aulanni'am Aulanni'am
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Diabetes mellitus type 1 (DMT1) is an insulin deficiency disorder caused by pancreatic damage characterized by chronic hyperglycemia. Animal modeling of DMT1 using inducting Multiple Low Dose Streptozotocin (MLD-STZ) elevat levels of free radicals and inflammation in pancreatic organs which then damage β cells. Ruellia tuberosa L. is a plant containing triterpenoids from the class of antioxidant and anti-inflammatory flavonoids. This study used three groups of rat: negative control group, positive control and 240 mg/kg bw therapy group. Study are based on changes MDA levels, blood glucose and histologic of pancreas. MDA level determining with TBA method, glucose levels using EasyTouch GCU and histopalogy of pancreas using Hematoxylin-Eosin staining. Statistical analysis of MDA and blood glucose (α = 0.05) showed significant decreases in MDA and blood glucose levels. Based on the results of histologic observations of pancreas descriptively showed a decreas in cell damage on Langerhans island. Thus, the provision of root extract of Ruellia tuberosa L. can inhibit the damage in rat DMT1 model so that it can be used as therapy of diabetes mellitus.

In Vitro and In Silico Study of Immobilized Xylanase from Trichoderma viride using Bentonite Matrices Activated with HCl Nur Lailah; Sasangka Prasetyawan; Arie Srihardyastutie
The Journal of Pure and Applied Chemistry Research Vol 6, No 2 (2017): Edition of May-August 2017
Publisher : Chemistry Department, The University of Brawijaya

The purpose of this study was aimed determine the optimum conditions of agitation time and concentration of immobilized xylanase by in vitro and in silico and efficient reuse of immobilized xylanase. The results of in silico test showed that xylan bounded on the residues of xylanase active site through hydrogen bonding with the amino acid glutamic 177A, glutamate 86A, tyrosine 88A, arginine 122A, and glutamine 136A, whereas bentonite and the residues of xylanase active site form hydrogen bonding with the amino acid glutamine 52, arginine 81, isoleucine 128 and glycine 130. The results of in vitro study showed that the optimum condition of xylanase immobilization was achieved at agitation time of 3 hours with the amount of xylanase adsorbed was 12.593 mg/g bentonites and activity of 50.328 units and the concentration of immobilized xylanase of 4.259 ppm and the amount of xylanase adsorbed of 16.162 mg/g bentonites and activity of 56.362 units. The immobilized xylanase can be used as many as five repetitions with the residual enzyme activity of 60%.

Co-Authors Achmad Rudijanto Agustina Shinta Hartati Wahyuningtyas Akhmad Sabarudin Alyaa Farrah Dibha Andreas Budi Wijaya Angelina Rosmawati Ani Mulyasuryani Anisa Aprilia Anna Roosdiana Anna Roosdiana Anna Roosdiana Anna Roosdiana Asyfariatus Zulfa Azhar Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulanni`am Aulanni`am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am, Aulanni’am Azor Yulianus Tefa Barlah Rumhayati Barlah Rumhayati Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Danar Purwonugroho Danar Purwonugroho Destyana Ellingga Pratiwi Destyana Ellingga Pratiwi Devi Ramadani Diah Mardiana Dolly Irnawati Neno Dyah Kinasih Wuragil Endah Setiani Astuti Eny Rahma Wati Eva Nur Laili Octaviana Fahmi, Ahmad Luthfi Fahriyah Fahriyah Faijal Fachrudin Mbabho Fiddaroini, Saidun Firza Rajasa Gunawan Fitria Rahmawati Hani Susanti, Hani Janatun Na’imah Karti'a, Galuh Wahyu KIKI FIBRIANTO Latifah Tribuana Dewi Luluk Indri Astuti Made Oka Adnyana Made Oka Adnyana Maria Natalia Wewo Masruri Masruri Moh. Farid Rahman Muhammad Asadullah Nabila, Sofi Naila Azmi Adiba Nur Aeni Nur Lailah Nur Samsu Pertiwi, Viâ€™in Ayu Pertiwi, Vi’in Ayu Putranty Widha Nugraheni Qonitah Fardiyah Riawati Riawati Riyanti Isaskar Roja’i, Abdul Munir Rulli Rosandi Rurini Retnowati Safitri, Anna Salsabila, Isadora Evani Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Siti Nurul Afifah Sofi Nabila Sutrisno Sutrisno Tri Ardiyati Tri Ardyati Velayaty Labone Azzahra Vi’in Ayu Pertiwi Warsito Warsito Warsito Warsito Yudita Prihatini Puji Rahma Sari Yulianti Antula Yuniar Ponco Prananto Yusri Fajar

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