PCJN Pharmaceutical and Clinical Journal of Nusantara
Vol. 4 No. 02 (2026): PCJN: Pharmaceutical and Clinical Journal of Nusantara

Antioxidant Activity Assessment and Profiling of Total Phenolic and Flavonoid Contents in Bay Leaf Extract (Syzygium polyanthum Folium)

Hutagaol, Cindy Jesika (Unknown)
Yunus, Muhammad (Unknown)
Piska, Finna (Unknown)



Article Info

Publish Date
31 Mar 2026

Abstract

Bay leaf (Syzygium polyanthum (Wight) Walp.) is a traditional medicinal plant containing phenolic and flavonoid compounds with potential antioxidant properties. Free radicals contribute to various degenerative diseases, highlighting the need for safe and effective natural antioxidants. This study aimed to evaluate the antioxidant activity of ethanolic extract of bay leaves using the DPPH method and to determine its total phenolic and flavonoid contents. This experimental laboratory study employed maceration extraction using 96% ethanol. Antioxidant activity was assessed by the DPPH radical scavenging assay at 517 nm and expressed as IC₅₀ value. Total phenolic content was determined using the Folin–Ciocalteu method at 765 nm and expressed as mg gallic acid equivalents (GAE)/g extract, while total flavonoid content was measured using the AlCl₃ colorimetric method at 430 nm and expressed as mg quercetin equivalents (QE)/g extract. The results showed that the ethanolic extract exhibited a strong antioxidant activity with an IC₅₀ value of 33.43 ppm. The total flavonoid content was 14.4992 mg QE/g extract (1.4499%) with excellent linearity (r² = 0.99747), while the phenolic calibration curve showed good linearity (r² = 0.99045). In conclusion, the ethanolic extract of S. polyanthum leaves demonstrates strong antioxidant activity supported by its phenolic and flavonoid contents, indicating its potential as a natural antioxidant source.

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Journal Info

Abbrev

pcjn

Publisher

Subject

Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

Description

Journal that publishes Focus & Scope research articles, which include: Pharmaceutical Biology Pharmaceutical Technology Pharmaceutical Chermistry Pharmacology Clinical Pharmacy Microbiology Pharmacy This journal is published by the Nusantara Scientific Journal , which is published March, July, and ...