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Piska, Finna
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Inhibisi Enzim α-Glukosidase oleh Bacillus Megaterium ITU 9 Isolat Sumber Air Panas Sumatera Utara Piska, Finna; Ginting, Chrismis Novalinda; Harmileni, Harmileni; Fachrial, Edy
Jurnal Pharmascience Vol 11, No 1 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.17045

Abstract

Diabetes adalah penyakit multifaktor yang insidensinya meningkat dengan pesat di negara berkembang. Diabetes mellitus (DM) tipe 2 adalah jenis diabetes yang paling umum ditemui, dimana penyakit ini erat kaitannya dengan kemajuan industri dan teknologi sehingga mempengaruhi gaya hidup seseorang. Penanggulangan penyakit ini dapat dilakukan dengan menghambat aktivitas α-glukosidase. Beberapa mikroorganisme terutama dari sumber air panas dapat dimanfaatkan sebagai penghasil senyawa inhibitor α-glukosidase. Pada penelitian sebelumnya isolat Bacillus megaterium ITU 9 merupakan isolat yang diisolasi dari sumber air panas. Pada penelitian ini diuji kemampuan isolat Bacillus megaterium ITU 9 sebagai penghasil inhibitor α-glukosidase dengan metode sumur. Daya persen inhibisi supernatan B. megaterium ITU 9 terhadap kerja enzim α-glukosidase adalah sebesar 46,67%, sedangkan daya persen inhibisi acarbose yaitu 70%. Dalam hal ini B. megaterium ITU 9 termofilik memiliki daya inhibisi terhadap enzim α-glukosidase. Kata Kunci: Bakteri Termofilik, Inhibitor, Akarbose, Metode Sumur, Persen Inhibisi Diabetes is a multifactorial disease whose incidence is increasing rapidly in developing countries. Diabetes mellitus (DM) type 2 is the most common type of diabetes, where this disease is closely related to industrial and technological advances, thus affecting a person's lifestyle. This disease can be controlled by inhibiting α-glucosidase activity. Several microorganisms, especially from hot springs, can be used to produce α-glucosidase inhibitor compounds. In previous research, the Bacillus megaterium ITU 9 isolate was an isolate isolated from hot springs. In this study, the ability of the Bacillus megaterium ITU 9 isolate to produce α-glucosidase inhibitors was tested using the well method. The percent inhibition power of B. megaterium ITU 9 supernatant on the action of the α-glucosidase enzyme was 46.67%, while the percent inhibition power of acarbose was 70%. In this case, the thermophilic B. megaterium ITU 9 has inhibitory power against the α-glucosidase enzyme.
Antioxidant test of crude extract of secondary metabolites from Fusarium sp. isolate of rizosphere of cat’s whisker Piska, Finna; Zega, Sati Angriani; Hutauruk, Rico Halim; Septia, Evianti Dwi; Meutia, Rena
Jurnal Prima Medika Sains Vol. 6 No. 2 (2024): December
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v6i2.5344

Abstract

Antioxidants are compounds needed in the prevention and therapy of diseases such as diabetes mellitus, cancer and premature aging. Over time, secondary metabolite compounds as antioxidants can be obtained from microbes, namely those originating from the endophyte and rhizosphere of plants. Based on previous research, secondary metabolites that act as antioxidants are flavonoids and phenolics, with phenolic compounds and flavonoids can bind free radicals through hydroxyl groups present in their aromatic rings. Microbes that live on medicinal plants will have similarities in producing secondary metabolites which can also be used as medicine. Fusarium sp. Laboratorium Basic Science UNPRI (LBSU) isolate is an isolate isolated from the rhizosphere of the cat's whisker plant (Orthosiphon stamineus). The cat's whisker plant is known to be an antioxidant. This study aims to test the antioxidant crude extract of secondary metabolites from Fusarium sp. LBSU. The method for isolating secondary metabolites is by liquid fermentation, then extracted using ethyl acetate and tested for antioxidant activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The antioxidant test results were obtained from the DPPH radical inhibition value by Ethyl acetate Extract of Fusarium sp. LBSU (EEF) is 44%, while Vitamin C is 88%. Thus, the ability to capture DPPH free radicals by EEF is 44%, weaker than Vitamin C.
Antibacterial test of secondary metabolites of Rhizosphere bacterial isolates of cat whisker against Escherichia coli and Staphylococcus aureus Septia, Evianti Dwi; Hutauruk, Rico Halim; Razoki, Razoki; Piska, Finna
Jurnal Prima Medika Sains Vol. 6 No. 2 (2024): December
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v6i2.5544

Abstract

The rhizosphere is the zone between the root surface and soil. Rhizosphere bacteria can produce metabolites that are antibacterial because they produce phytotherapeutic compounds that have the same properties as those produced by the plant. The purpose of this study was to isolate secondary metabolite compounds from the rhizosphere bacteria of cats’ whisker plants (Orthosiphon stamineus) as antibacterial agents against Escherichia coli and Staphylococcus aureus. This study began with the isolation of rhizosphere bacteria, purification, bacterial characterization, bacterial fermentation, and antibacterial tests. The results of the isolation of Orthosiphon stamineus rhizosphere bacteria obtained as many as six isolates, with each isolate coded as RKK-1, RKK-2, RKK-3, RKK-4, RKK-5, and RKK-6. The results showed that RKK-5 had an inhibition zone against Escherichia coli with a diameter of 8.5 mm. Based on these results, it can be concluded that RKK-5 has a moderate response to Escherichia coli.
Antibacterial Activity of the Secondary Metabolite of Fusarium LBSU Isolate from the Cat’s Whiskers Plant (Orthosiphon stamineus) Zega, Sati Agriani Zega; Halim, Rico; Ginting, Astri Natalia; Piska, Finna
Jurnal Ners Vol. 9 No. 3 (2025): JULI
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v9i3.46029

Abstract

The cat's whisker plant is known to contain bioactive metabolites that have potential as therapeutic agents, while its rhizosphere is a habitat for microorganisms that produce secondary metabolites. Fusarium LBSU isolate were isolate from the rhizosphere of cat’s whiskers plant. Secondary metabolites are produced through liquid fermentation, followed by extraction using ethyl acetate. Antibacterial activity was tested using the disk diffusion method against the Escherichia coli and Staphylococcus aureus. The highest antibacterial activity obtained against Escherichia coli 23.565 mm, higher than Staphylococcus aureus which is 15.3 mm. These findings support the further development of secondary metabolites from rhizosphere isolate as an alternative source of environmentally friendly and effective antibacterial agents.
Co-Authors Edy Fachrial Ginting, Astri Natalia Ginting, Chrismis Novalinda Halim, Rico Harmileni Hutauruk, Rico Halim Meutia, Rena Razoki, Razoki Septia, Evianti Dwi Zega, Sati Agriani Zega Zega, Sati Angriani