Effect of Gracilaria verrucosa Extract as an Immunostimulant on the Non-Specific Immune System of Striped Catfish (Pangasius hypophthalmus)

Arif Habib Fasya (Study Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine, and Life Sciences, Airlangga University, Banyuwangi, Indonesia)
Laksmi Sulmartiwi (Marine Department, Faculty of Fisheries and Marine, Airlangga University, Surabaya 60115, Indonesia)
Prayogo (Study Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine, and Life Sciences, Airlangga University, Banyuwangi, Indonesia)
Hairul Hafiz Bin Mahsol (Conservation Biology Program, Faculty of Tropical Forestry, University Malaysia Sabah, Kinabalu, Malaysia)
Siska Adelia Sitompul (Study Program of Aquaculture, Department of Health and Life Sciences, Faculty of Health, Medicine, and Life Sciences, Airlangga University, Banyuwangi, Indonesia)

Journal of Aquaculture Science
Vol 10 No 2 (2025): Journal of Aquaculture Science

One of the failures of freshwater fish farming is often caused by the attack of A. hydrophila bacteria. This bacterial attack can be prevented by administering immunostimulants derived from G. verrucosa extract. This study aims to determine the non-specific immune response of striped catfish to Aeromonas hydrophila infection following supplementation of G. verrucosa extract in feed. Five doses were administered in this study: 1 ml, 1.5 ml, and 2 ml per kg of feed, as well as a positive control and a negative control. Blood samples were collected every week after infection, and the parameters observed were total erythrocyte and leucocyte counts, as well as leucocyte differential counts. The results showed that G. verrucosa extract significantly increased blood cell counts. The highest dose was found to be the most effective in increasing the non-specific immune response of striped catfish against A. hydrophila infection