Aquatic environments contaminated with iron can pose a threat to human health and other organisms, with a threshold concentration of 0.2 mg/L for hygiene and sanitation purposes. This study aims to analyze Fe(III) concentrations in water using UV-Vis spectrophotometry with a natural complexing agent from flamboyant flower extract as a more economical and environmentally friendly alternative to KSCN. Flamboyant flower extract contains anthocyanin compounds with a density of 1.02 g/cm³ that form a red-colored complex with Fe(III) and exhibit maximum absorption at a wavelength of 510 nm. Optimal complex formation conditions were achieved at pH 1 with an extract volume of 0.15 mL and a reaction time of at least 40 minutes. The complex was found to be stable for 2 days. Analysis results showed that the Fe(III) concentration in the tested water sample was 1.09 mg/L.
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