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Journal of Microbiology, Biotechnology and Conservation
Published by Universitas Mataram
ISSN : -     EISSN : 3090708X     DOI : https://doi.org/10.29303/jmbc.v1i2
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology
The journal prioritizes papers in the following areas: Basic and Applied Microbiology Biotechnology and its applications in various aspects of life Bioinformatics Microbiome Molecular Biology and Genetics Conservation of Biota and Environment All organisms related to microbial communities, including fungi, plants, animals, humans, and viruses In line with the multidisciplinary nature of microbiology, biotechnology, and conservation.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 30 Documents
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Anatomi Organ Vegetatif Tumbuhan Rija-Rija (Scleria melaleuca) di Kawasan Hutan Kemasyarakatan (HKM) Pusuk Lestari, Lombok Barat Adinda Emilia Sajida; Baiq Kesy Juliani; Baiq Zulifa Hemidia; Siti Rizki Fitri; R. Muhamad Azis Maulana; Kurniasih Sukenti; Tri Mulyaningsih
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.5998

Abstract

Cyperaceae is a plant species that belongs to the sedges family and is characterised by a triangular stem. The sedges plant is frequently observed in rice fields and under the shade of trees, including Scleria melaleuca.The objective of this study is to ascertain the anatomical characteristics of S. melaleuca.The study employed a descriptive exploratory research method and was conducted at the Advanced Biology Laboratory, Faculty of Mathematics and Natural Sciences, University of Mataram. The S. melaleuca plant samples were collected from the Hutan Kemasyarakatan (HKM) Pusuk Lestari Area in Batu Layar, West Lombok Regency, West Nusa Tenggara. The samples included the root organs, stems, leaves and seeds. The samples were preserved in 70% alcohol and sliced using the hand-free section method. The results of the study demonstrated that the S. melaleuca exhibited distinctive characteristics, including the presence of bulliform cells between the leaf epidermis, the presence of both long and short cells, closed concentric vascular bundles in the stems, a triangular stem shape, and amphicribal vascular bundles in the roots.
Karakterisasi Hama Baru Spodoptera Frugiperda Pada Tanaman Jagung (Zea mays L.) Di Lombok Barat Erwin Juwanda; Bambang Supeno; Hery Haryanto; Muhammad Hari Aditia Pratama
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6062

Abstract

Maize (Zea mays L.) is the main food crop in Indonesia after rice, but is vulnerable to attack by plant pests (OPT), including Spodoptera frugiperda, an invasive insect that attacks corn plants. This research aims to observe the population, level of attack and characteristics of the pest of S. frugiperda in West Lombok Regency. Based on observations, the eggs of this pest are brownish white, round in shape, and have an average size of 0.33 mm × 0.47 mm. The male pupa is reddish brown with dimensions of 14.25 mm × 4.32 mm, while the female pupa is larger, namely 17.53 mm × 5.13 mm. Larvae are found in various instars (2-6) with sizes from 1.53 mm to 35.16 mm, and colors varying from light green to dark brown. Male imagos are smaller than females, with a length of 12.45 mm and a wingspan of 27.73 mm, while female imagos have a size of 14.38 mm and a wingspan of 31.47 mm. The highest population was found at location 3 with 123 larvae, while the lowest population was at location 13 with 1 larva. The highest attack rate occurred at location 7 (50%) and the lowest at location 11 (5%). The observation method includes assessing the level of damage based on the attack intensity score (0–4). This research proposes further studies to understand the distribution and control mechanisms of S. frugiperda in order to minimize its impact on corn cultivation, especially in Nusa Tenggara Barat, Indonesia.
Spider Diversity in Potato Plants Applied with Some Concentrations of Botanical Pesticides Virginia Tobacco Stem Waste Lalu Ahmad Fauzan; Muhammad Sarjan; Bambang Supeno; Muhammad Hari Aditia Pratama
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6071

Abstract

This study aims to determine the effect of applying various concentrations of botanical pesticides derived from Virginia tobacco stem waste on the diversity of spiders in potato plants. The research was conducted in Sembalun Bumbung Village, Sembalun District, East Lombok Regency, West Nusa Tenggara Province. The method employed was an experimental method with field trials, using a Randomized Block Design (RBD) with six treatments: P0 (control), P1 (abamectin), P2 (Virginia tobacco stem extract 2 ml/L), P3 (Virginia tobacco stem extract 4 ml/L), P4 (Virginia tobacco stem extract 6 ml/L), and P5 (Virginia tobacco stem extract 8 ml/L). The experiment was conducted in three blocks, resulting in 18 experimental units. The results showed that the application of various concentrations of botanical pesticides from Virginia tobacco stem waste did not significantly affect spider diversity in potato plants. However, the P3 treatment (Virginia tobacco stem extract 4 ml/L) exhibited a higher tendency in population, diversity index, abundance index, and dominance index compared to other treatments.
Penampilan Karakter Agronomi Beberapa Genotipe Mutan Padi (Oryza Sativa L.) Baas Selem Generasi Kedua (M2) Hasil Induksi Mutasi Faihrorrozy; Ni Wayan Sri Suliartini; I Ketut Ngawit; Muhammad Hari Aditia Pratama
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6080

Abstract

This study aims to determine the differences in the appearance of agronomic characters of several genotypes of rice mutants (Oryza Sativa L.) The second generation Baas Selem (M2) is the result of mutation induction. This research was conducted in June 2021 - October 2021 in Nyiur Lembang Village, Narmada District, West Lombok Regency. The methods used were group random design (RAK) and Augmented Design with the treatment used, namely 24 genotypes of second generation Baas Selem rice seeds (M2) with control plants. The control plant repeated 3 times, while the mutant plant was 1 time, in its application using an augmented design. The data obtained were analyzed using ANOVA and BNT at the level of 5%. The results showed that Baas Selem rice mutants differed markedly in all observed agronomic characters, from plant height, flag leaf area, total number of tillers, number of productive tillers, number of grain contained, number of empty grains, weight of grain contained, weight of empty grain, weight of grain 100 seeds, flowering age of harvest age and panicle length.
Sifat Mekanik Edible Film Berbasis Protein dengan Penggunaan Konsentrasi Plasticizer Sorbitol yang Berbeda Fitri; Fahrullah; Wahid Yulianto
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6107

Abstract

Edible film is a type of biodegradable packaging that can be an alternative to food packaging material to reduce environmental damage and maintain food product’s shelf life, quality, and safety. This research aims to determine the effect of the mechanical properties of protein-based edible film using different concentrations of sorbitol plasticizer on edible film thickness, gelation time, and film microstructure. To make edible films, the research material consists of sorbitol, whey protein, gelatin, distilled water, and silica gel. The research was conducted using a completely randomized design with three sorbitol plasticizer treatments consisting of P1:35%, P2:40%, P3:45%, and three replications. The data were analyzed using Analysis of Variance (ANOVA) and continued with the Duncan Multiple Range Test (DMRT) using the SPSS program. The research results showed that the use of sorbitol plasticizer with different concentrations gave a very significant difference (P<0.01) to P1 and P3, but P2 was not significantly different from the thickness of the edible film. The gelation time did not make a difference (P>0, 05), which is real, but adding different sorbitol to the edible film microstructure can produce varying microstructures. From the research results, the average values for edible film thickness are 0.053, 0.078, and 0.113 mm, while the gelation time produces average values of 19.33, 13.33, and 19 minutes. This research concludes that using different sorbitol concentrations provides a very significant difference in film thickness; however, unlike the gelation time, which does not make a difference, the microstructures produce varying microstructures.
Keanekaragaman Artropoda Predator pada Tanaman Kentang (Solanum tuberosum L.) yang Diaplikasikan Pestisida Nabati Batang Tembakau dan Mimba Rizky, M. Muslihan; Sarjan, Muhammad; Stella Petrunella Thei, Ruth
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 2 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i2.6111

Abstract

This study aims to determine the diversity, evenness, dominance and abundance of predatory arthropods in potato plants that have been applied with plant pesticides from tobacco stems and neem. This research was conducted in June-August 2022 on land owned by farmers in the Sembalun Bumbung area, Sembalun District. This study used an experimental method with field and laboratory experiments. The experimental design used in this study was RAK (Randomized Block Design) with 4 treatments (control, chemical pesticide abamectin, tobacco pesticide and neem pesticide) with 4 repetitions. Observations in the field were carried out 10 times with an interval of 1 time a week. The results of this study found 26 species of predatory arthropods belonging to 18 Families, 7 Orders and 2 Classes. The total number of individual predatory arthropods found was 662 individuals. The value of the diversity index (H') ranges from 1.59-1.88 which is classified as moderate. The value of the € evenness index ranges from 0.49-0.59 which is quite even. Dominance index values (D) ranged from 0.21-0.31 which indicated that no single species was too dominating. Abundance index (K) values ranged from 14,898-32,345 with the highest abundance in the neem plant pesticide treatment. The results of the ANOVA test analysis showed that all treatments had significant differences, so a BNJ (Honest Significant Difference) follow-up test was carried out with a level of 5%. Further test results showed no significant differences in diversity, evenness and dominance, but significantly different in abundance
Pengaruh Pestisida Nabati Limbah Batang Tembakau Virginia terhadap Serangan Hama Pengisap Daun (Thrips Palmi) Pada Tanaman Kentang (Solanum tuberosum L.) Adhia Nirmala, Wiwin; Sarjan, Muhammad; Haryanto, Hery
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 2 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i2.6112

Abstract

This study aims to determine the effect of several concentrations of botanical pestisides from virginia tobacco stem waste to the leaf-sucking pests (Thrips palmi) on potato plants. This exteriment was carried out from August to November 2020 in Sembalun Bumbung Village, Sembalun District, East Lombok Regency, West Nusa Tenggara. The method use in this study is an experimental method with field experiments and the design used is a Rendomized Block Design (RBD) with 6 treatments, P0 (control), P1 (abamectin), P2 (botanical pesticide of Virginia tobacco stem waste 2 ml/L), P3 (botanical pesticide of Virginia tobacco stem waste 4 ml/L), P4 (botanical pesticide of Virginia tobacco stem waste 6 ml/L) and P5 (botanical pesticide of Virginia tobacco stem waste 8 ml/L). each treatment had 3 replications so that 18 experimental plots were obtained. The result showed that the concentration of Virginia tobacco stem pesticide was more effective in controlling the populations of Thrips palmi than other Virginia tobacco stem pesticide concetrations. However, the Virginia tobacco stem waste botanical pesticide was not effective on controlling the intensity of T. palmi
Pengaruh Pestisida Nabati Limbah Batang Tembakau Virginia Terhadap Keragaman Kumbang Koksi Sebagai Predator Hama Pada Tanaman Kentang (Solanum Tuberosum L.) Maulana Aziz, Lutfi; Sarjan, Muhammad; Haryanto, Hery
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 2 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i2.6113

Abstract

This study aims to determine the effect of vegetable pesticides from virginia tobacco stem waste on the diversity of the koksi beetle predators on potato plants. The method used is an experimental method with direct experiments in the field. The experimental design used was a randomized block design (RAK) with 6 treatments, namely P0 (control), P1 (abamectin), P2 (vegetable insecticide of Virginia tobacco stem waste 2 ml/l), P3 (vegetable insecticide of Virginia tobacco stem waste 4 ml/l). l), P4 (vegetable insecticide of Virginia tobacco stem waste 6 ml/l), and P5 (vegetable insecticide of Virginia tobacco stem waste 8 ml/l). This research was conducted in Sembalun Bumbung Village, Sembalun District, East Lombok Regency, West Nusa Tenggara from August 2020 to November 2020. The results showed that treatment at the concentration of P5 (vegetable pesticide from virginia tobacco stem waste 8 ml/l) showed a suppression value. The diversity and abundance index of the predators of the koksi beetle were the most influential compared to the treatment of several concentrations of other vegetable pesticides, while the application of botanical pesticides with various concentrations was not able to suppress the predatory population of the koksi beetle in the species Cheliomenes sexmaculata and Coccinella transveralis and was able to suppress the population of Coleophora inaqualis. Cheilomenes sexmaculata species has the highest population compared to the other 2 species.
Potensi Anti Xanthomonas dari Bakteri Rizosfer Tanaman Kankung Pagar (Ipomoea carnea) Nurmaliana Asmayani; Tri Wahyu Setyaningrum; A.A. Ngurah Nara Kusuma; Nur Indah Julisaniah; Muhammad Hari Aditia Pratama; Wanda Qoriasmadillah; Faturrahman
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6159

Abstract

The plant pathogenic bacterium Xanthomonas campestris is a Gram-negative, rod-shaped bacterium that causes damage to plant leaves. This particular disease can result in significant economic losses and a reduction in the quality of agricultural products. An alternative method of dealing with this bacteria is to utilise the rhizosphere of kale (Ipomoea carnea) as a biological control. The objective of this study was to ascertain the antibacterial potential of the rhizosphere bacteria of kale (Ipomoea carnea) against the Xanthomonas campestris bacteria and to identify isolates with enhanced antibacterial efficacy against Xanthomonas campestris using the well diffusion method. A total of 13 bacterial isolates were obtained from the rhizosphere of kale, exhibiting distinctive round colonies, flat edges, and a white or cream colouration. A total of 12 gram-negative isolates and one gram-positive isolate were identified. The highest inhibitory activity against X. campestris was demonstrated by isolates R1, R3, and R11, with an average inhibitory zone diameter of 20.01 mm, 20.79 mm, and 20.57 mm, respectively. These values fell into the very strong inhibition category.
Analysis of Land Cover Change in Aik Berik Village Area, Central Lombok Regency Anis, Anis Syakiratur Rizki; Mulyaningsih, Tri; Propita Lestari, Dinda; Primaditya Hasanto, Rizki
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 2 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i2.6202

Abstract

Land cover change in tourist areas is often an important issue related to the impact of human activities on the environment. This study aims to analyze land cover changes in the Aik Berik Village area, Central Lombok Regency, by utilizing remote sensing technology and Geographic Information System (GIS). Multitemporal satellite image data is used to identify types of land cover, such as forests, agricultural land, built land, and open land, over a period of time. Spatial analysis is carried out to determine the pattern of change and the factors that affect it. The results of the analysis showed that there was a significant change in land conversion, with the conversion of forest areas into plantations, rice fields, and built-up land. Despite this, most of the forest area has been preserved thanks to natural regeneration factors and community-based management. In addition, some plantation areas have changed their function to rice fields and built land, while savanna areas are relatively stable. These changes have the potential to affect the quality of ecosystems and the hydrological cycle of the region. The success of Aik Berik Village in maintaining forests and springs that are vital to the community shows the importance of sustainable and community-based land management.

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