Biodiversitas Journal of Biological Diversity
Vol. 16 No. 2 (2015)

Assessment of genetic diversity among soursop (Annona muricata) populations from Java, Indonesia using RAPD markers

SURATMAN SURATMAN (Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
ARI PITOYO (Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
SRI MULYANI (Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)
SURANTO SURANTO (Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University)



Article Info

Publish Date
19 Sep 2015

Abstract

Suratman, Ari Pitoyo, Sri Mulyani, Suranto. 2015. Assessment of genetic diversity among soursop (Annona muricata)populations from Java, Indonesia using RAPD markers. Biodiversitas 16: 247-253. The objective of this study was to determine geneticdiversity of the soursop (Annona muricata L.) populations from Java (Indonesia) using RAPD markers. A total of 70 individuals werecollected from 7 soursop populations, distributed along the geographical range of natural distribution in Java. Genetic diversity wasestimated by RAPD technique using 6 arbitrary selected primers. Those primers produced 151 polymorphic bands with the percentageof polymorphism for each primer ranged from 95% to 100%. The genetic diversity value (h) within each population ranged from 0.0418to 0.0525. The highest h value (0.0525) was found in the KRA population whereas the lowest h value was observed in the PCTpopulation. The highest genetic distance value (0.0410) was observed in SKH-GKD populations pair whereas the lowest geneticdistance value (0.02448) was estimated in KRA-PCT populations pair. Based on the dendogram, the seven soursop populations weresegregated into three major clusters. The first cluster consisted of SKH, KRA, PCT, NGW, and KPG populations. The GKD populationwas then grouped into second cluster. In the third cluster, the BGR population was grouped separately and more genetically distant thanthe others. However, the relationship dendrogram showed that the grouping did not always indicate the geographical origins similarity,but possibly showed the genetic similarity.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...