Biodiversitas Journal of Biological Diversity
Vol. 19 No. 3 (2018)

Characterizations of endophytic Bacillus strains from tomato roots as growth promoter and biocontrol of Ralstonia solanacearum

YULMIRA YANTI (Department of Plant Protection, Faculty of Agriculture, Universitas Andalas. Limau Manih, Pauh, Padang 25163, West Sumatra, Indonesia.)
WARNITA WARNITA (Department of Agronomy, Faculty of Agriculture, Universitas Andalas. Padang 25163, West Sumatra, Indonesia)
REFLIN REFLIN (Department of Plant Protection, Faculty of Agriculture, Universitas Andalas. Limau Manih, Pauh, Padang 25163, West Sumatra, Indonesia.)
CHAINUR RAHMAN NASUTION (Postgraduate Program, Department of Plant Protection, Faculty of Agriculture, Universitas Andalas. Padang 25163, West Sumatra, Indonesia)



Article Info

Publish Date
01 May 2018

Abstract

Yanti Y, Warnita, Reflin, Nasution CR. 2018. Characterizations of endophytic Bacillus strains from tomato roots as growth promoter and biocontrol of Ralstonia solanacearum. Biodiversitas 19: 906-911. Bacterial wilt caused by Ralstonia solanacearum is the most damaging vascular pathogens in tomato and many other crops in tropical, subtropical and warm temperate areas of the world limiting its production. Biological agents such as Plant growth Promoting Rhizobacteria (PGPR) is considered as a potential biological control agent for the suppression of plant diseases such as bacterial wilt. Bacillus spp. are one of the most potential genera of PGPR group used for controlling pathogens and promoting plant growth because of their spore-forming ability which increases their adaptation to the environment. The aims of the research were to isolate Endophytic Bacillus isolates, to characterize its ability as plant growth promoter and pathogen controller, and to identify its molecular genetic using 16S rRNA. Bacillus strains were isolated from healthy tomato roots. All Bacillus spp. strains acquired from isolation were then screened directly on plants in completely randomized design experiments with 3 replications. All potential strains were screened and identified using 16S rRNA with 27F and 1492R primers. Results showed that out of 15 obtained isolates, 6 of them showed a good ability to both promote growth and control R. solanacearum. All isolates were identified as B. Pseudomycoides strain NBRC 101232, B. cereus strain CCM 2010, B. toyonensis strain BCT-7112, B. anthracis strain ATCC 14578, B. cereus strain JCM 2152 and B. cereus ATCC 14579.

Copyrights © 2018






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...