Biodiversitas Journal of Biological Diversity
Vol. 19 No. 5 (2018)

Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers

AIDA FITRIANI SITOMPUL (Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. Jl. Willem Iskandar, Pasar V, Medan Estate, Medan 20221, North Sumatra, Indonesia.)
ELIDA HAFNI SIREGAR (Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. Jl. Willem Iskandar, Pasar V, Medan Estate, Medan 20221, North Sumatra, Indonesia.)
DEWI IMELDA ROESMA (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas. Kampus Unand Limau Manih, Padang 26253, West Sumatra, Indonesia.)
DAHELMI DAHELMI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas. Kampus Unand Limau Manih, Padang 26253, West Sumatra, Indonesia.)
EKO PRASETYA (Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. Jl. Willem Iskandar, Pasar V, Medan Estate, Medan 20221, North Sumatra, Indonesia.)



Article Info

Publish Date
21 Sep 2018

Abstract

Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers. Biodiversitas 19: 1877-1883. Coffee (Coffea arabica L.) is one of the most important economic commodities in the province of North Sumatra, Indonesia. Insects associated with pollination of C. arabica are one of the key factors for successful cultivation of C. arabica, but, the research regarding of these was still limited. The population of coffee plant is scattered across the highlands of Indonesia and the pollination of C. arabica is strongly believed linked to a diverse group of pollinating insects. However, lack of taxonomic identification of insects pollinating these plants has become one of constraints to succeed the cultivation of C. Arabica. This study aimed to analyze types and variations of pollinating insects of C. arabica in the province of North Sumatra, Indonesia, using DNA barcoding. DNA barcoding is now considered an alternative method of molecular identification. Sixteen of C. arabica flower visitors were captured in different planting location in North Sumatra province. Using mtDNA markers, the cytochrome oxidase subunit sequence I (COI), about 12 pollinator insect species were identified based on the COI sequence i.e Amegilla cingulata, Apis dorsata, Apis cerana, Trigona chanchamayoensis, Idiella divisa, Dolichopodidae sp., Allactoneura sp., Stomorhina discolor, Phytomia erratica, Rhiniidae sp., Melipona bicolor, and Hymenoptera sp.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...