Short Communication: Update phylogenetic information of the local varieties of cloves (Syzygium aromaticum) from North Maluku, Indonesia based on ITS sequences data

SUNDARI SUNDARI (Department of Biology Education, Faculty of Teacher Training and Education, Universitas Khairun. Jl. Pertamina Kampus II Unkhair Gambesi, Ternate 97728, North Maluku, Indonesia)
NURHASANAH NURHASANAH (Department of Biology Education, Faculty of Teacher Training and Education, Universitas Khairun. Jl. Pertamina Kampus II Unkhair Gambesi, Ternate 97728, North Maluku, Indonesia)
ABDU MAS’UD (Department of Biology Education, Faculty of Teacher Training and Education, Universitas Khairun. Jl. Pertamina Kampus II Unkhair Gambesi, Ternate 97728, North Maluku, Indonesia)
MOHAMAD AMIN (Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang. Jl. Semarang No. 5, Sumbersari, Malang City 65145, East Java, Indonesia)
ESTRI LARAS ARUMINGTYAS (Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran 5, Sumbersari, Lowokwaru, Malang City 65145, East Java, Indonesia)
RODIYATI AZRIANINGSIH (Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran 5, Sumbersari, Lowokwaru, Malang City 65145, East Java, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 20 No. 6 (2019)

Abstract. Sundari, Nurhasanah, Mas'ud A, Amin M, Arumingtyas EL, Azrianingsih R. 2019. Update phylogenetic information of the local varieties of cloves (Syzygium aromaticum) from North Maluku, Indonesia based on ITS sequences data. Biodiversitas 20: 1510-1515. Clove (Syzygium aromaticum L. Merr & Perry) is a native spice plant species in Indonesia, especially Maluku and North Maluku. The phylogenetic relationship among local varieties of cloves in North Maluku, Indonesia is currently unclear; therefore molecular verification research is required for further conservation and breeding programs. This study aims to identify the taxonomic position and phylogenetic relationship among two valuable local varieties of cloves rom North Maluku, i.e., ’Afo’ and ’Sibela’, based on ITS sequences data. Afo clove is considered as the oldest clove native to Ternate island, and Sibela clove is the oldest clove native to Bacan Island. The phylogenetic analysis using both Maximum Parsimony and Neighbour Joining algorithms confirmed the monophyletic group of cloves (S. aromaticum) and separated to outgroup of Eugenia species. Both phylogenetic trees produced three clades with the same pattern and supported by strong bootstrap values of 99-100%.