Biodiversitas Journal of Biological Diversity
Vol. 21 No. 5 (2020)

The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil

Nita Shilfiani Rohmah Nita Shilfiani Rohmah (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia)
SUHARJONO (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia)
YOGA DWI JATMIKO (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia)
DIAN SISWANTO (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia)
IRFAN MUSTAFA (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia)



Article Info

Publish Date
13 Apr 2020

Abstract

Abstract. Rohmah NS, Suharjono, Jatmiko YD, Siswanto D, Mustafa I. 2020. The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil. Biodiversitas 21: 1920-1927. Endophytic bacteria tolerant of chromium have the potential to reduce Cr (VI) to Cr (III) and produce compounds that can improve plant survival in Cr (VI) contaminated soils. This study was performed to isolate and identify Cr (VI)-tolerant bacteria from the endosphere of Ficus septica Burm.F growing on the tannery waste contaminated soil, Screening of bacteria was carried out based on the level of Cr (VI) tolerance. High tolerant isolates were tested for their potential of Indole Acetic Acid (IAA) and exopolysaccharide (EPS) production, phosphate solubilizing, and the presence of chromate reductase (ChR) genes. The most potential bacterial isolate was identified based on 16S rDNA sequences. The results showed that three isolates (E3, E4, and E8) were tolerant of Cr (VI) up to 900 mg/L. E8 isolates produced the highest IAA production (20.05 ?g/mL), phosphate solubilization (127.78 ?g/mL), and EPS production (9,70 ?g/mL), and all isolates had the ChR gene. E8 isolate was found to be 100% identical to Enterobacter cloacae ATCC 13047.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...