Assessment of zygosity and nitrogen used efficacy in Mekongga transgenic rice

MACHZANI QURAITUL AINI (Biotechnology Program, Graduate School, Institut Pertanian Bogor. Jl. Raya Dramaga, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia)
SUHARSONO SUHARSONO (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia)
ANIVERSARI APRIANA (Research Center for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency. Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia)
ATMITRI SISHARMINI (Research Center for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency. Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia)
TRI JOKO SANTOSO (Research Center for Horticulture and Estate Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency. Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor 16915, West Java, Indonesia)
KURNIAWAN RUDI TRIJATMIKO (International Rice Research Institute. 10th Floor Suite 1009, Security Bank Center 6776 Ayala Avenue, Makati Metro Manila 1226, Philippines)

Biodiversitas Journal of Biological Diversity
Vol. 23 No. 8 (2022)

Abstract. Aini MQ, Suharsono, Apriana A, Sisharmini A, Santoso TJ, Trijatmiko KR. 2022. Assessment of zygosity and nitrogen used efficacy in Mekongga transgenic rice. Biodiversitas 23: 4040-4046. Nitrogen (N) is a vital macronutrient that can be a limiting factor for rice plant growth. Nitrogen-efficient plants can be assembled by modifying nitrogen-related metabolic pathways to improve Nitrogen Use Efficiency (NUE) in plants. T0 generation of transgenic Mekongga rice plants containing the LeAlaAT gene was obtained. In transgenic plant research, zygosity test was desirable to ensure the integration and inheritance stability of transgene. This study aimed to assess zygosity and nitrogen use efficacy of two transgenic Mekongga rice lines, namely M41 and M50. This research involved three main activities, i.e. detection of flanking sequence for T0 generation by TAIL-PCR method, zygosity test for T1 generation, and efficacy test for T2 generation of Mekongga transgenic lines in response to different N fertilizer rates of 0, 60, 90, and 120 kg ha?1. Zygosity analysis showed that six out of 14 M41 and three of 13 M50 transgenic lines were homozygous for LeAlaAT transgene. The efficacy test showed that homozygous transgenic lines had higher tiller numbers, grain numbers, and biomass compared to non-transgenic ones under the same nitrogen level. The notable discrepancies of the transgenic plants can also be recognized in the absorption, agronomical, and grain NUE of the studied plants.