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All Journal Biodiversitas Journal of Biological Diversity
ATMITRI SISHARMINI
Research Center for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency. Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Assessment of zygosity and nitrogen used efficacy in Mekongga transgenic rice MACHZANI QURAITUL AINI; SUHARSONO SUHARSONO; ANIVERSARI APRIANA; ATMITRI SISHARMINI; TRI JOKO SANTOSO; KURNIAWAN RUDI TRIJATMIKO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230823

Abstract

Abstract. Aini MQ, Suharsono, Apriana A, Sisharmini A, Santoso TJ, Trijatmiko KR. 2022. Assessment of zygosity and nitrogen used efficacy in Mekongga transgenic rice. Biodiversitas 23: 4040-4046. Nitrogen (N) is a vital macronutrient that can be a limiting factor for rice plant growth. Nitrogen-efficient plants can be assembled by modifying nitrogen-related metabolic pathways to improve Nitrogen Use Efficiency (NUE) in plants. T0 generation of transgenic Mekongga rice plants containing the LeAlaAT gene was obtained. In transgenic plant research, zygosity test was desirable to ensure the integration and inheritance stability of transgene. This study aimed to assess zygosity and nitrogen use efficacy of two transgenic Mekongga rice lines, namely M41 and M50. This research involved three main activities, i.e. detection of flanking sequence for T0 generation by TAIL-PCR method, zygosity test for T1 generation, and efficacy test for T2 generation of Mekongga transgenic lines in response to different N fertilizer rates of 0, 60, 90, and 120 kg ha?1. Zygosity analysis showed that six out of 14 M41 and three of 13 M50 transgenic lines were homozygous for LeAlaAT transgene. The efficacy test showed that homozygous transgenic lines had higher tiller numbers, grain numbers, and biomass compared to non-transgenic ones under the same nitrogen level. The notable discrepancies of the transgenic plants can also be recognized in the absorption, agronomical, and grain NUE of the studied plants.
Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance NURUL FARIDAH RIFHANI; ANIVERSARI APRIANA; ATMITRI SISHARMINI; TRI JOKO SANTOSO; KURNIAWAN RUDI TRIJATMIKO; INEZ H. SLAMET-LOEDIN; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 24 No. 6 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240620

Abstract

Abstract. Rifhani NF, Apriana A, Sisharmini A, Santoso TJ, Trijatmiko KR, Slamet-Loedin IH, Yunus A. 2023. Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance. Biodiversitas 24: 3258-3268.Rice cv. Mentik Wangi is a local aromatic rice that is susceptible to pests and diseases, such as bacterial leaf blight (BLB) caused by Xanthomonas oryzae(Xoo). This bacteria can cause damage to plants thereby reducing crop yields. This study aimed to obtain the CRISPR/Cas9 module construct as well as introducing the construct into rice cv. Mentik Wangi for developing BLB resistance. The manufacture of the CRISPR/Cas9 module was carried out using the Golden Gate method, and the introduction of that construct into rice cv. Mentik Wangi was carried out using Agrobacterium tumefaciens. Constructing a CRISPR/Cas9 module with multiple gRNA of OsSWEET11 and OsSWEET14 genes has succeeded, 129 putative transformant lines of T0 generation produced with the regeneration and transformation efficiency values ??are respectively 9.4% and 9.8%. The results showed that 36 lines were positive for the hpt gene, indicating that the CRISPR/Cas9-gRNAOsSWEET module construct was successfully entered into rice cv. Mentik Wangi. Further analysis is needed to identify mutagenesis in the target gene region of TI generation transgenic plants as well as phenotypic tests for BLB resistance.
Evaluating the phenotypic responses of CRISPR/Cas9-edited Mentik Wangi rice mutants T1 focusing on GA20ox2 and OsCKX2 gene knockout TILMIIDZAH SALMA FATHIN; ATMITRI SISHARMINI; ANIVERASRI APRIANA; TRI JOKO SANTOSO; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 25 No. 8 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250852

Abstract

Abstract. Fathin TS, Sisharmini A, Apriana A, Santoso TJ, Yunus A. 2024. Evaluating the phenotypic responses of CRISPR/Cas9-edited Mentik Wangi rice mutants T1 focusing on GA20ox2 and OsCKX2 gene knockout. Biodiversitas 25: 2783-2790. Mentik Wangi is a local Indonesian rice variety with tall plant stature, making it susceptible to lodging and yield loss, necessitating trait improvements to overcome these weaknesses. Gene editing of GA20ox2 and OsCKX2 using CRISPR/Cas9 was conducted to improve the characteristics of Mentik Wangi. This study aimed to evaluate the phenotypic responses of T1 Mentik Wangi mutant lines generated through gene editing using the CRISPR/Cas9 system. Eighty-five mutant lines and wild-type plants were grown and observed in a greenhouse. Phenotypic traits were observed as quantitative traits, such as plant height, panicle length, total number of tillers, number of productive tillers, total number of grains, 1000-grain weight, heading date, and harvesting age; and qualitative traits, such as panicle type and panicle exsertion type. The results showed a decrease in plant height and panicle length in Mentik Wangi rice mutant lines. Several lines exhibited improvements in total tiller number, productive tiller number, total grain number, and 1000-grain weight. Mutant lines required longer to reach the generative phase and displayed differences in panicle type and panicle exsertion type. This study reported that the T1 mutant lines demonstrated distinct phenotypic responses indicating improvements in the desired traits.
Co-Authors AHMAD YUNUS AHMAD YUNUS ANIVERASRI APRIANA ANIVERSARI APRIANA INEZ H. SLAMET-LOEDIN KURNIAWAN RUDI TRIJATMIKO MACHZANI QURAITUL AINI NURUL FARIDAH RIFHANI SUHARSONO SUHARSONO TILMIIDZAH SALMA FATHIN TRI JOKO SANTOSO TRI JOKO SANTOSO