Biodiversitas Journal of Biological Diversity
Vol. 23 No. 12 (2022)

Microbial exploration from two different ecosystems in Central Sulawesi, Indonesia

RATNAWATI RATNAWATI (Department of Agrotechnology, Faculty of Agriculture, Universitas Alkhairaat. Jl. Diponegoro No.39, Lere, Palu Barat, Palu, Central Sulawesi 94221, Indonesia)
SRI SUDEWI (Department of Agrotechnology, Faculty of Agriculture, Universitas Alkhairaat. Jl. Diponegoro No.39, Lere, Palu Barat, Palu, Central Sulawesi 94221, Indonesia)
KASMAN JAYA (Department of Agrotechnology, Faculty of Agriculture, Universitas Alkhairaat. Jl. Diponegoro No.39, Lere, Palu Barat, Palu, Central Sulawesi 94221, Indonesia)
ABDUL RAHIM SALEH (Department of Agrotechnology, Faculty of Agriculture, Universitas Sintuwu Maroso. Jl. Ir. P. Timor 01, Poso 94612, Central Sulawesi, Indonesia)



Article Info

Publish Date
27 Dec 2022

Abstract

Abstract. Ratnawati, Sudewi S, Jaya K, Saleh AR. 2022. Microbial exploration from two different ecosystems in Central Sulawesi, Indonesia. Biodiversitas 23: 6191-6198. A supportive growing environment and the presence of microbes in the soil are considered good habitats for plant growth. Endophytic microbes live in symbiosis (mutual benefit) with their host plants, both in the stem and root tissues of plants, providing defense services in biotic and abiotic stress situations. This study was aimed to identify various microbes that exist in various agricultural cultivation ecosystems in Central Sulawesi. Microbial sampling was obtained from healthy plant tissues (endophytes) and rhizosphere in lowland rice and shallot plantation ecosystems. The sample obtained was then weighed as much as 5 g and surface sterilized for further isolation by serial dilution method to be spread on NA (Nutrient Agar) media for bacteria, and PDA (Potato Dextrose Agar) for fungi. The microbes that had grown were re-isolated to obtain pure isolate culture of microbes. The results showed that 8 isolates from the rhizosphere and 4 isolates as endophytes in the ecosystem of organic rice fields were recorded. The fungal isolates from the shallot plantation “Lembah Palu” found as many as 12 isolates from rhizosphere and 4 isolates as endophytes. The results of macroscopic and microscopic characteristics on fungal isolates identified as the species of Trichoderma, Fusarium, Penicillium, Gliocladium and Aspergillus.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...