Isolation and identification of fungi associated with Falcataria moluccana gall-rust in Kediri, East Java, Indonesia

YUNIK ISTIKORINI (Department of Silviculture, Faculty of Forestry and Environment, Institut Pertanian Bogor. Jl. Ulin Lingkar Akademik, Darmaga, Bogor 16680, West Java, Indonesia)
NOFITA NUR CAHYANI (Department of Silviculture, Faculty of Forestry and Environment, Institut Pertanian Bogor. Jl. Ulin Lingkar Akademik, Darmaga, Bogor 16680, West Java, Indonesia)
NOOR FARIKHAH HANEDA (Department of Silviculture, Faculty of Forestry and Environment, Institut Pertanian Bogor. Jl. Ulin Lingkar Akademik, Darmaga, Bogor 16680, West Java, Indonesia)
HASYYATI SHABRINA (Forestry Program, Faculty of Agriculture, Universitas Mataram. Jl. Pendidikan No. 37, Mataram 83351, West Nusa Tenggara, Indonesia)
ADITYA NUGROHO (Department of Silviculture, Faculty of Forestry and Environment, Institut Pertanian Bogor. Jl. Ulin Lingkar Akademik, Darmaga, Bogor 16680, West Java, Indonesia)
FITRI INDRIANI (Research Center for Plant Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency. Jl. Ir. H. Juanda No. 18, Bogor 16122, West Java, Indonesia)
ULFAH JUNIARTI SIREGAR (Department of Silviculture, Faculty of Forestry and Environment, Institut Pertanian Bogor. Jl. Ulin Lingkar Akademik, Darmaga, Bogor 16680, West Java, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 23 No. 12 (2022)

Abstract. Istikorini Y, Cahyani NN, Haneda NF, Shabrina H, Nugroho A, Indriani F, Siregar UJ. 2022. Isolation and identification of fungi associated with Falcataria moluccana gall-rust in Kediri, East Java, Indonesia. Biodiversitas 23: 6171-6179. Gall-rust disease caused by Uromycladium falcatariae is the most important disease of sengon in Indonesia. Plant pathogenic parasitism as a biological control mechanism is usually associated with fungal biocontrol agents. This study aimed to isolate and identify the fungi associated with the sengon gall rust from the Forest Management Unit of Jatirejo, Indonesian State Forest Company, Kediri. Fungi were isolated from the black or gray-green patches of gall-rust samples on Potato Dextrose Agar (PDA) plates. The fungi were identified using macroscopic and microscopic examinations. The molecular identification of fungal DNA was amplified by PCR using a specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in GenBank (NCBI). DNA barcode identification results showed 9 isolates containing of 8 species, namely Fusarium oxysporum, F. solani, F. chlamydosporum, F. circinatum, F. incarnatum, F. verticillioides, Lasiodiplodia theobromae, and Pestalotiopsis palmarum. This result showed that the Fusarium was the most fungi commonly found fungi on the gall's rust teliospore surface.