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All Journal Jurnal Silvikultur Tropika (JST) Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Hutan Tropika Jurnal Pusat Inovasi Masyarakat Jurnal Sylva Lestari
Istikorini, Yunik
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Materials Science & Nanotechnology Social Sciences

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Potensi Cendawan Endofit pada Tanaman Binahong (Anredera cordifolia (Ten.) Steenis) untuk Mengendalikan Botryodiplodia theobromae Pat. Penyebab Mati Pucuk pada Bibit Jabon (Anthocephalus cadamba (Roxb.) Miq) Yunik Istikorini
Jurnal Silvikultur Tropika Vol. 10 No. 2 (2019): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.10.2.114-118

Abstract

Endophytic fungi provides potential advantages as biocontrol agents in the plant. Binahong (Anredera cordifolia) is a medicinal plant which can potentially overcome many kinds of diseases. The dieback disease is caused by Botryodiplodia theobromae Pat. that may lead the death of the host. The objective of this research was to analyze the potency of endophytic fungi from binahong leaves to control the fungus B. theobromae as causal agent of dieback disease on jabon (Anthocepalus cadamba). The isolates of endophytic fungi from binahong used were bnh1.1, bnh1.2, bnh1.3, bnh4.2, bnh4.5, bnh3.3 and bnh3.4. The antifungal effect of endophytic fungi againts colony growth of virulent B. theobromae were tested in vitro. The results showed that all endophytic fungus (7 isolates) inhibited the colony development of B. theobromae ranging from 28.52%-52.22%. Treatment with endophytic fungi bnh4.5 and bnh4.2 had protected jabon from virulent B. theobromae. It has been expressed by the delay of the incubation period and the decreasing of disease severity (respectively 33.34% and 40.84%. Key words: Anredera cordifolia, Botryodiplodia theobromae, disease incidence, endophytic fungi
UJI KESEHATAN BENIH KENANGA YLANG-YLANG: Cananga odorata Lam. Hook.f. & Thomson) forma genuina (Health test of Kenaga Ylang-Ylang Seed (Cananga odorata Lam. Hook.f. & Thomson) forma genuina) Yunik Istikorini; Arum Sekar Wulandari; Wahyu Krisna
HUTAN TROPIKA Vol 15 No 2 (2020): Volume 15 Nomor 2 Tahun 2020
Publisher : Jurusan Kehutanan, Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jht.v15i2.2159

Abstract

Ylang-ylang (Cananga odorata Hook.F.& Thomson) is one of the defense plants with various benefits and advantages. One of the hallenges in ylang-ylang Cananga cultivation is the contribution of high-quality seed. The study aimed to determine the health of seeds and identify fungi carried by ylang-ylang seeds. The seed health test used an incubation method on potato dextrose agar (PDA) media. Results showed that ylangylang seeds associated with five fungal for 11 months of age and newly harvested seedswere the same fungal. Those isolated fungal were Aspergillus niger, A. flavus, Penicillium sp., Botryodiplodia sp. and Paecilomyces sp. The fungus A. niger is the dominant fungus. Fungi Paecilomyces sp. and Botryodiplodia sp. The fungus A. niger identified most frequently. Fungi Paecilomyces sp. and Botryodiplodia sp. had a fast growth rate, whereas Penicillium sp. had slower growth compared with other isolates. Keywords: Fungus, seed health, ylang-ylang seeds
Identification of Endophytic Fungi of Balangeran (Shorea balangeran Korth.) by Morphological Characterization Yunik Istikorini; Okta Yulia Sari
Jurnal Sylva Lestari Vol. 10 No. 2 (2022): May
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v10i2.547

Abstract

Endophytic fungi are the potential biological agent that could stimulate plant growth and inhibit plant disease. The existence of diverse and abundant endophytic fungi encourages continuous exploration. One of the plants that have potential as a source of endophytic fungi is balangeran (Shorea balangeran Korth.). The study aimed to identify the species of endophytic fungi from the leaf and twig of S. balangeran based on morphological characteristics. Fungi isolation was conducted by multiple sterilizations and purification. Furthermore, the macroscopic and microscopic morphological characteristics of the endophytic fungi isolates were also identified. The results showed nine colony characteristics of endophytic fungi. Endophytic fungi of Colletotrichum sp1. (SbD 1.1), Phomopsis sp. (SbD 1.3.1), Colletotrichum sp2. (SbD 1.3.2), and Beauveria sp. (SbD 3.1) were only found on the leaves, while Aspergillus niger (SbB 5.1), Colletotrichum sp3. (SbB 5.2), and Nigrospora sp. (SbB 5.3.2 and SbB 6.3) were only found on the twigs. Endophytic fungi Phyllosticta sp. (SbD 1.2) were found in the leaves and twigs. The growth rate of endophytic fungi showed that Colletotrichum sp3 (SbB 5.2) was the fastest, and Phyllosticta sp. (SbD 1.2) was the slowest among the nine isolates. Endophytic fungi that have been isolated will be analyzed for their benefits as a biological agent in future research. Keywords: endophytic fungi, identification, morphological characterization, Shorea balangeran
Effect of Endophytic Fungi on the Growth of Sengon (Falcataria moluccana) Yunik Istikorini; Ahmad Faqih; Noor Farikhah Haneda; Ulfah Juniarti Siregar; Dimas Andrianto
Jurnal Sylva Lestari Vol. 10 No. 3 (2022): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v10i3.550

Abstract

Endophytic fungi have been reported to promote plant growth and resistance to biotic and abiotic stresses. This study aimed to determine the effect of endophytic fungi application isolated from the sengon (Falcataria moluccana) seeds on the growth of sengon. The seed-borne endophytic fungi were applied when the session was three weeks old by sprinkling each suspension of endophytic fungi (103 to 106 spores/ml) on the soil around the plant roots. The experimental parameters evaluated were height, diameter, and total wet and dry weight. This study showed that endophytic fungi treatment has no significant effect on the stem diameter, total wet weight, and water content; but significantly affected the height and total dry weight. Cladophialophora sp. and Ascotricha sp. fungi increased the height growth of sengon, while Aspergillus sp. fungi increased the total dry weight against the control. Ochroconis sp. treatment was not significantly different from the control. The shoot-root ratio values range from 1.13-1.43, indicating that the seeds already have roots supporting plant growth. The Dickson quality index range from 0.65-1.03 suggested that the seedlings have good biomass distribution. Consequently, endophytic fungi have the potential to increase the growth of sengon seedlings. Keywords: endophytic fungi, Falcataria moluccana, seedling growth, sengon
Effect of Endophytic Bacteria Inoculation and Eco-enzyme on Kayu Kuku (Pericopsis mooniana Thw.) Seedlings Growth Arum Sekar Wulandari; Yunik Istikorini; Yus Septiawati
Jurnal Silvikultur Tropika Vol 14 No 01 (2023): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.14.01.15-24

Abstract

Improving the physical quality of kayu kuku seedlings can be done by applying endophytic bacteria and ec-enzymes. The aims of this study were to examine (1) the effect of inoculation of endophytic bacteria on the germination of kuku wood seeds, and (2) the effect of inoculation and application of eco-enzymes on the growth of kuku wood seedlings. The endophytic bacteria were isolated from the shoot of Shorea balangeran plant with the codes SBP 1, SBP 2, and SBP 19. Bacterial application was conducted on seeds and seedlings, while eco-enzyme application was only conducted on kayu kuku seedlings. The results of the research at the seed level showed that inoculation of the endophytic bacteria SBP 19 could accelerate the germination rate of kayu kuku seeds. At the seedling level, inoculation of endophytic bacteria SBP 1, SBP 2, SBP 19 and application of eco-enzymes had no significant effect on height, diameter, number of new leaves, total fresh weight and total dry weight of kayu kuku seedlings. The shoot root ratio of kayu kuku seedlings ranged from 2.66-5.04 and the seedlings quality index ranged from 0.41-0.93, indicating that the seedlings were ready to be transplanted into the field. In general, inoculation of endophytic bacteria had a significant effect on kayu kuku seed germination, but had not been able to increase the growth of kayu kuku seedlings during the 14 weeks of observation. Keywords: Pericopsis mooniana Thw., seed dormancy, seed germination, seedling quality index, shoot root ratio
Pelatihan Pembuatan Pupuk Hayati pada Sistem Agroforestri berbasis Kopi di Desa Garahan, Jember, Jawa Timur Yunik Istikorini; Muhammad Alam Firmansyah; Lufthi Rusniarsyah; Ikhwan Shodiq; Tiska Aulia Azzahra; Idelia Latifah
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 2 (2023): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.9.2.191-198

Abstract

The productivity of coffee plants on the most cultivated land in Garahan Village decreased. This decline is thought to be caused by, among others, climate change, land degradation and the presence of pests and diseases in coffee plants. This training aims to introduce pests and diseases in coffee-based agroforestry to farmers and develop environmentally friendly biofertilizers. This activity begins with a pre-test, socialization and training and ends post-test. Based on the survey and direct observation, coffee plants found upas fungal diseases, root diseases, and stem borer pests. The agroforestry system applied is complex and straightforward agroforestry. In complex agroforestry, coffee plants are grown with various other crops, such as pepper, vanilla, leucaena, taro, banana, gamal, and mahogany. Simple agroforestry consists of coffee land planted with single-shade crops such as leucaena and gamal or a combination of Leucaena and pine. The training results showed an increased farmer's better understanding of coffee pests and diseases, the benefits of agroforestry, and the manufacture of biofertilizers. The biofertilizer developed is expected to increase the productivity of coffee plants.
Potential Test of Rizosphere Microbes as Biological Controls that Cause Plant Diseases Istikorini, Yunik; Budiman, Thurfah
Jurnal Silvikultur Tropika Vol 14 No 03 (2023): Journal of Tropical Silviculture
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.14.03.242-249

Abstract

Biological control is an alternative to reducing the use of pesticides because it can suppress the growth of plant pathogens and has no negative impact on the environment. This study aimed to test the effectiveness of rhizosphere microbes as biological controllers that cause plant diseases. This study contains three rhizosphere microbes (Trichoderma sp., Gliocladium sp., Bacillus sp.) and three pathogenic test fungi (Rhizoctonia sp., Sclerotium sp., Botryodiplodia sp.) were used. This study was divided into three experiments, namely (1) an in vitro antagonist test, (2) a secondary metabolite test, and (3) a growth test on sengon seeds (in vivo). The parameters measured were percentage inhibition, germination rate, growth rate, disease incidence, and disease intensity. The fungus Trichoderma sp. and Gliocladium sp. can inhibit the growth of pathogenic fungi Sclerotium sp., Rhizoctonia sp., and Botryodiplodia sp. ranging from 61.82% to 80.00%. Secondary metabolites of the fungus Gliocladium sp. are more able to inhibit the mycelia growth of pathogenic fungi Rhizoctonia sp., Sclerotium sp., and Botryodiplodia sp. compared to the fungus Trichoderma sp. with inhibition values were 71.13%, 33.83%, 23.58%, respectively. Gliocladium sp., Trichoderma sp., and Bacillus sp. can suppress disease incidence (14.29%, 12.38%, and 15.24%) and intensity of damping-off attacks caused by Rhizoctonia sp. (8.91%, 8.57%, and 9.43%). This shows that biological agents have the potential to control plant diseases Keywords: Bacillus aereus, damping-off, secondary metabolites, Trichoderma
Pelatihan Pembuatan Pupuk Hayati di Desa Gondel, Kecamatan Kedungtuban, Kabupaten Blora, Jawa Tengah Yunik Istikorini; Muhammad Alam Firmansyah; Fitrianingrum Kurniawati; Nadzirum Mubin
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 3 (2024): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.10.3.305-314

Abstract

The rice productivity in Gondel Village, Kedungtuban District, Blora Regency, Central Java, has declined. Factors such as attacks by plant pests and diseases (OPT), climate change (DPI) impact, and decreasing soil quality are the leading causes of crop failure. This training aims to enhance the understanding and skills of Gondel Village farmers in making biofertilizers and introducing environmentally friendly pest and disease control methods. The activity involved 40 farmers from 8 farmer groups (Gemah Ripah, Margo Mulyo, Ngudi Makmur, Sido Dadi, Sido Makmur, Tambah Makmur, Tambah Rizki, and Sri Mulyo), with each group represented by five farmers. The training methods included analysis of soil chemicals, socialization, practical propagation of Trichoderma sp. on rice media, and biofertilizer production. Pre-test and post-tests were conducted to assess the improvement in farmers' knowledge. Trichoderma biofertilizer was formulated using carriers such as a) Manure and b) Compost, zeolite, and humic acid. The results of the soil chemical properties analysis showed that farmers' use of chemical fertilizers was very intensive, resulting in very high levels of phosphorus (P) and calcium (Ca) and high levels of potassium (K), magnesium (Mg), and cation exchange capacity (KTK). Evaluation results indicated that the training significantly improved the farmers' knowledge of biofertilizer production in theory and practice. However, some farmers still need further guidance on the propagation of the biological agent Trichoderma sp.
Application of Manure and Eco-Enzymes on Eucalyptus in Clay Mines at PT. Solusi Bangun Indonesia Istikorini, Yunik; Mansur, Irdika; Padilah, Sinta Nur
Jurnal Silvikultur Tropika Vol 15 No 03 (2024): Journal of Tropical Silviculture
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.15.03.228-233

Abstract

Pertambangan tanah liat secara terbuka berpotensi mengganggu ekosistem lingkungan. Reklamasi dilakukan untuk membantu pemulihan lingkungan yang terganggu akibat proses pertambangan. Penelitian ini bertujuan mengetahui pengaruh pemberian pupuk kandang dan ekoenzim terhadap pertumbuhan tanaman kayu putih di lahan pasca tambang tanah liat serta mendapatkan dosis kombinasi pupuk kandang dan ekoenzim yang sesuai untuk meningkatkan pertumbuhan tanaman kayu putih di lahan pasca tambang tanah liat. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 2 faktor yaitu pupuk kandang (0, 1, 1,5 kg) dan ekoenzim (0, 100, 150 ml). Hasil penelitian menunjukkan bahwa interaksi antara pupuk kandang dan ekoenzim terdapat pengaruh nyata terhadap tinggi dan ketebalan tajuk tanaman, namun kedua perlakuan tidak berpengaruh nyata terhadap diameter dan diameter tajuk tanaman. Dosis terbaik untuk meningkatkan pertumbuhan kayu putih di lahan pasca tambang tanah liat adalah 150 ml ekoenzim. Kata kunci: ekoenzim, kayu putih, pupuk kandang, reklamasi, tambang tanah liat.
Viabilitas Benih Mindi (Melia Azedarach L.) Melalui Uji Daya Hantar Listrik Febrina Keumala, Cut; Sekar Wulandari, Arum; Istikorini, Yunik
Journal of Tropical Silviculture Vol. 16 No. 01 (2025): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.16.1.28-32

Abstract

Penyimpanan benih dalam jangka waktu tertentu dapat memengaruhi viabilitas benih. Tujuan penelitian ini adalah menganalisis pengaruh lama penyimpanan benih terhadap viabilitas benih mindi. Metode yang digunakan adalah uji daya hantar listrik atau electrical conductivity (EC) terhadap air rendaman benih yang telah disimpan selama 0, 2, 4, 6, dan 8 bulan. Pengukuran nilai konduktivitas listrik dilakukan menggunakan alat conductivity meter. Hasil penelitian menunjukkan bahwa lama penyimpanan memengaruhi nilai EC benih mindi. Nilai EC terendah adalah 15,63 µS/cm pada penyimpanan 0 bulan dan nilai EC tertinggi adalah 31,87 µS/cm pada penyimpanan 8 bulan. Lama penyimpanan memiliki korelasi positif terhadap nilai EC sebesar 0,75. Nilai EC yang tinggi menunjukkan banyaknya perembesan cairan sel yang keluar dari benih sehingga menurunkan kapasitas perkecambahan benihnya. Nilai EC memiliki korelasi negatif terhadap viabilitas benih. Secara umum, semakin lama benih disimpan, maka viabilitas benih semakin menurun.
Co-Authors Ahmad Faqih Alam, Ilham Fatkhy Ardian, Dava Putra Arum Sekar Wulandari Budiman, Thurfah DIMAS ANDRIANTO Febrina Keumala, Cut Fiqri, Muhammad Fitrianingrum Kurniawati, Fitrianingrum Fortunata, Maura Rezky Idelia Latifah Ikhwan Shodiq Irdika Mansur Lufthi Rusniarsyah Maulina, Alaysha Muhammad Alam Firmansyah Nadzirum Mubin Navizan, Fadhli Zhafran Noor Farikhah Haneda Okta Yulia Sari Padilah, Sinta Nur Rohmah, Sabila Nur Sari, Okta Yulia Sutanto, Vallari Kalea Ananda Tiska Aulia Azzahra Ulfah Juniarti Siregar Wahyu Krisna Yus Septiawati