Biodiversitas Journal of Biological Diversity
Vol. 24 No. 3 (2023)

Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic

TETTY MARTA LINDA (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)
BERNADETA LENI FEBRIARTI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)
DELITA ZUL (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)
NERY SOFYANTI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)
AZIZUL BERLIANSYAH (Laboratory of Microbiology and Bioprocess, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)
NOVIZA DELFIRA (Laboratory of Biochemistry Bioprocess, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)
SILVERA DEVI (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. Subrantas Km. 12,5 Simpang Baru, Pekanbaru 28293, Riau, Indonesia)



Article Info

Publish Date
23 Mar 2023

Abstract

Abstract. Linda TM, Febriarti BL, Zul D, Sofyanti N, Berlyansah A, Delfira N, Devi S. 2023. Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic. Biodiversitas 24: 1580-1588. Endophytic bacteria are found in symbiotic plant tissues. These bacteria pose potential sources for the natural product due to their health benefit, e.g., for antidiabetic activity. The present study aimed to isolate and characterize endophytic bacteria isolates from the sterile leaf of sea fern (Acrostichum aureum Linn.) from Bengkalis Island (Riau Province), Indonesia. Furthermore, to investigate their potency as an antidiabetic agent. The characteristics of isolates bacteria were described based on colony morphology and biochemical tests. A total of six endophytic bacteria were isolated from the sterile leaf of Acrostichum aureum. Their characteristics vary in shape, color, and size. The phytochemical screening indicated the presence of alkaloids and saponin in all the isolates. The results exhibited that the extract of the sterile leaf of A. aureum also gave a similar phytochemical compound of isolate endophytic bacteria. The dendrogram constructed from morphological and biochemical characteristics showed the separation of D.SB 4.2 from other six bacteria strains due to the positive antibacterial activity for Escherichia coli ATCC35218, Staphylococcus aureus ATCC29213, Salmonella typhi ATCC14028, and Bacillus subtilis ATCC11774. Out of six isolates, only isolate D.SB 5.2 was selected for the antidiabetic test. The isolates showed positive results on the activity as a-amylase inhibitor (18.12%). This study provides important information on endophytic bacteria isolates on A. aureum and their potency for antidiabetic agents.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...