Biodiversitas Journal of Biological Diversity
Vol. 24 No. 1 (2023)

Detection of methicillin-resistant Staphylococcus aureus and multidrug resistance isolated from cats in animal clinic at Sidoarjo District, East Java, Indonesia

YUSAC KRISTANTO KHODA WARUWU (Profession Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
ASWIN RAFIF KHAIRULLAH (Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
MUSTOFA HELMI EFFENDI (Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
BAMBANG SEKTIARI LUKISWANTO (Department of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
DANIAH ASHRI AFNANI (Master Program in Veterinary Disease and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
SHENDY CANADYA KURNIAWAN (Master program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen 6708 PB, Netherlands)
OTTO SAHAT MARTUA SILAEN (Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia. Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia)
KATTY HENDRIANA PRISCILIA RiwuRIWU (Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia. Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia)
AGUS WIDODO (Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia. Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia)
SANCAKA CHASYER RAMANDINIANTO (Lingkar Satwa Animal Care Clinic. Jl. Sumatera No. 31L, Gubeng, Surabaya 60281, East Java, Indonesia)



Article Info

Publish Date
18 Jan 2023

Abstract

Abstract. Waruwu YKK, Khairullah AR, Effendi MH, Lukiswanto BS, Afnani DA, Kurniawan SC, Silaen OSM, Riwu KHP, Widodo A, Ramandinianto SC. 2023. Detection of methicillin-resistant Staphylococcus aureus and multidrug resistance isolated from cats in animal clinic at Sidoarjo District, East Java, Indonesia. Biodiversitas 24: 106-111. Methicillin-resistant Staphylococcus aureus is the name given to the strain of bacteria that is multidrug-resistant (MDR) and resistant to ?-lactam antibiotics. Comparatively to other livestock, companion animals have been highlighted more often as potential MRSA reservoirs. This study looked for MDR and cat-derived MRSA strains in Sidoarjo. One hundred cats with nasal swabs were accessed, along with the veterinary hospital and other clinics. Samples were collected using a sterile cotton swab and buffered peptone water as the transport medium, and then identified using a microbiological standard operating procedure. On five different antibiotic discs, the Kirby-Bauer diffusion method was used to determine the S. aureus antibiotic resistance profile. As a confirmatory test for MRSA, S. aureus isolates that were resistant to cefoxitin continued to grow on Oxacillin Resistance Screening Agar Base (ORSAB). Out of the seven MRSA isolates that were assumed to be MRSA, four of the seven MRSA isolates were confirmed to be MDR S. aureus. Humans and companion animals can both act as reservoirs for the recirculation of MRSA strains within the same household because cat nasal swabs resemble nosocomial MRSA and because both are more likely to get colonized than infected. It illustrated how cats might be a health risk to the public and acted as a cautionary tale about the inappropriate use of antibiotics.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...