Effect of a local foot-and-mouth disease vaccine candidate on TNF-α concentration and muscle necrosis in mice

Vengine Ulyama (Postgraduate student of Vaccinology and Immunotherapy, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia)
Fedik Abdul Rantam (Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia)
Vega Decline (Department of Animal Health, Faculty of Animal Science, Jambi University, Jambi, Indonesia)
Yulianna Puspitasari (Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia)
Hani Plumeriastuti (Department of Veterinary Pathology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia)
Jola Rahmahani (Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia)
Mohammad Anam Al Arif (Department of Livestock, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia)

ARSHI Veterinary Letters
Vol. 10 No. 2 (2026): ARSHI Veterinary Letters - May 2026

Foot-and-mouth disease (FMD) is a contagious transboundary disease that causes livestock losses. Vaccination is central to FMD control, but its effectiveness depends on the antigenic relevance to field strains. This study evaluated the safety and immunological response of a local isolate-based inactivated FMD vaccine candidate in mice. Eighty BALB/c mice were divided into four groups: adjuvant-only control, inactivated FMD virus antigen at 10⁸ TCID₅₀, 10⁷ TCID₅₀ with Montanide ISA, and 10⁶ TCID₅₀ with Montanide ISA. Immunization occurred intramuscularly on days 0 and 14. Tumor necrosis factor-alpha (TNF-α) concentrations were measured by ELISA, and injection-site tissues were examined histopathologically for necrosis. TNF-α concentrations increased after immunization, peaking post-booster in the adjuvanted 10⁷ TCID₅₀ group. Histopathology showed preserved architecture with no detectable necrosis. These findings suggest a systemic immune response without local muscle damage. Further studies in livestock are required to confirm protective efficacy, neutralizing antibody responses, and field-level safety.