INDUKSI KALUS EKSPLAN DAUN KOPI ROBUSTA DENGAN PENAMBAHAN BERBAGAI KONSETRASI 1-NAPHTHALENEACETIX ACID (NAA) SECARA IN VITRO: Induction of Robusta Coffee Leaf Explant Callus With the Addition of Various Concentrations 1-Naphthaleneacetix Acid (NAA) In Vitro

Ego Ego (Universitas Bangka Belitung)
Maera Zasari Zasari (Universitas Bangka Belitung)
Rosin Anika (Universitas Bangka Belitung)
Siti Khania (Universitas Bangka Belitung)
Okta Viola Sudanto (Universitas Bangka Belitung)
Jumita Jumita (Universitas Bangka Belitung)

ENVIAGRO
Vol 11 No 2 (2025): Enviagro

Increasing coffee growth can be done by inducing callus through in vitro culture propagation using Murashige and Skoog (MS) media with the addition of various concentrations of NAA. This research aims to determine the growth of coffee leaf callus explants in MS media with the addition of various concentrations of NAA in vitro and find out which concentration is best for the growth of coffee leaf callus explants in MS media in vitro. Coffee callus explants were cultured in MS media with the addition of various concentrations of NAA. This research used a randomized block design (CRD) and data was analyzed in percentage terms. The results of the research showed that the treatment of adding an NAA concentration of 2 ppm to MS media could increase the growth percentage of robusta coffee leaf callus explants. An NAA concentration of 2 ppm is the best concentration for culturally propagating coffee leaf callus explants in vitro.