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INDUKSI KALUS EKSPLAN DAUN KOPI ROBUSTA DENGAN PENAMBAHAN BERBAGAI KONSETRASI 1-NAPHTHALENEACETIX ACID (NAA) SECARA IN VITRO: Induction of Robusta Coffee Leaf Explant Callus With the Addition of Various Concentrations 1-Naphthaleneacetix Acid (NAA) In Vitro Ego Ego; Maera Zasari Zasari; Rosin Anika; Siti Khania; Okta Viola Sudanto; Jumita Jumita
Enviagro: Jurnal Pertanian dan Lingkungan Vol 11 No 2 (2025): Enviagro
Publisher : Program Studi S2 Ilmu Pertanian, Fakultas Pertanian, Fakultas Pertanian, Perikanan dan Biologi Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/3fj7sb64

Abstract

Increasing coffee growth can be done by inducing callus through in vitro culture propagation using Murashige and Skoog (MS) media with the addition of various concentrations of NAA. This research aims to determine the growth of coffee leaf callus explants in MS media with the addition of various concentrations of NAA in vitro and find out which concentration is best for the growth of coffee leaf callus explants in MS media in vitro. Coffee callus explants were cultured in MS media with the addition of various concentrations of NAA. This research used a randomized block design (CRD) and data was analyzed in percentage terms. The results of the research showed that the treatment of adding an NAA concentration of 2 ppm to MS media could increase the growth percentage of robusta coffee leaf callus explants. An NAA concentration of 2 ppm is the best concentration for culturally propagating coffee leaf callus explants in vitro.
RESPON PERTUMBUHAN BIBIT LADA DENGAN PERLAKUAN BERBAGAI KONSENTRASI BA (Benzyl Adenine): Growth Response of Pepper Seedlings to Various Concentrations of BA (Benzyl Adenine) Maera Zasari; Muniroh Muniroh; Andika Gus Manto Gus Manto; Aril Kurnata; Rina Tiwariska; Diana Chawla; Novita Sari; Jumita Jumita; Paskah Putra Pangihutan Hutasoit; Sarina Patima Patima; Sitti Nurul Aini
Enviagro: Jurnal Pertanian dan Lingkungan Vol 11 No 2 (2025): Enviagro
Publisher : Program Studi S2 Ilmu Pertanian, Fakultas Pertanian, Fakultas Pertanian, Perikanan dan Biologi Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ywgxef80

Abstract

Penelitian ini bertujuan untuk mengetahui respon pertumbuhan bibit lada (Piper nigrum L.) terhadap berbagai konsentrasi Benzyl Adenine (BA). Percobaan dilakukan menggunakan rancangan acak lengkap (RAL) dengan satu faktor, yaitu konsentrasi BA, yang terdiri atas beberapa taraf perlakuan dan diulang sebanyak tiga kali. Parameter yang diamati meliputi lebar daun, jumlah tunas, jumlah daun, dan tinggi tanaman. Hasil penelitian menunjukkan bahwa aplikasi BA berpengaruh nyata terhadap pertumbuhan vegetatif bibit lada. Konsentrasi BA 200 ppm menghasilkan pertumbuhan terbaik pada seluruh parameter, yaitu lebar daun tertinggi (±7–8 cm), jumlah tunas (±4–5 tunas), jumlah daun (±9–10 helai), dan tinggi tanaman (±15–17 cm). Hal ini menunjukkan bahwa BA pada konsentrasi optimal mampu merangsang pembelahan sel, pembentukan tunas lateral, dan pemanjangan batang tanpa menimbulkan efek toksik. Dengan demikian, konsentrasi BA 200 ppm direkomendasikan sebagai perlakuan paling efektif untuk meningkatkan pertumbuhan vegetatif bibit lada pada tahap pembibitan.  
Co-Authors Andika Gus Manto Gus Manto Aril Kurnata Diana Chawla Ego Ego Maera Zasari Maera Zasari Zasari Muniroh Muniroh Novita Sari Okta Viola Sudanto Paskah Putra Pangihutan Hutasoit Rina Tiwariska Rosin Anika Sarina Patima Patima Siti Khania Sitti Nurul Aini