Gourami (Osphronemus gouramy) farming faces challenges, including slow growth rates andhigh feed costs. This study aimed to evaluate the effects of probiotics, papain enzymes, andtheir combination on the growth performance and feed utilization of gourami. The study wasconducted using a completely randomized design with four treatments and three replicateseach. The treatments consisted of a control group (feed without papain enzyme or probiotics),treatment A (feed supplemented with 8 mL/kg probiotics), treatment B (feed supplementedwith 32.5 g/kg papain enzyme), and treatment C (feed supplemented with a combination of0.25 g/kg papain enzyme and 15 mL/kg probiotics). Juvenile gourami with average weight of5.82±0.09 g/fish were kept in nine fiberglass tank (3 m diameter and 1.2 m height) for 42 dayswith a stocking density of 15 fish/m2. After 42 days of rearing, treatment C exhibitedsignificantly higher specific growth rate, protein efficiency ratio, and protein retention than theother treatments (P<0.05), with values of 3.03±0.15%/day, 2.78±0.20%, and 44.71±3.06%,respectively. The lowest feed conversion ratio was also observed in treatment C, with a valueof 0.94±0.06 (P<0.05). In conclusion, supplementation with 0.25 g/kg papain enzyme and 15mL/kg probiotics in the feed enhanced growth performance and feed utilization in gourami.
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