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All Journal Jurnal Mina Sains Jurnal Ilmiah Samudra Akuatika ZIRAA'AH MAJALAH ILMIAH PERTANIAN Journal of Fisheries & Marine Jurnal Perikanan Journal of Vocational in Aquaculture (JAVA)
Muhammad Arif Mulya
Fish Hatchery Technology And Management, Vocational School, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Vibrio Dynamics and Health Status of Pacific White Shrimp Fed with Cinnamaldehyde-Containing Feed Dian Eka Ramadhani; Andri Hendriana; Dinamella Wahjuningrum; Muhammad Arif Mulya
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.34394

Abstract

Highlight Research Cinnamaldehyde for shrimp Dynamics of Vibrio and the correlation with shrimp health status Green and yellow Vibrio bacteria in water, intestine, and hepatopancreas of shrimp Cinnamaldehyde decrease the number of green Vibrio bacteria   Abstract Disease can cause shrimp mortality and high economic losses caused by Vibriosis group. One alternative is using cinnamaldehyde which function as an anti-microbial and inhibit the production of toxins released by microorganisms. This study aimed to evaluate the administration of cinnamaldehyde on the dynamics of Vibrio bacteria and the health status of Pacific white shrimp fed with cinnamaldehyde-containing feed. This study used a completely randomized design (CRD) consisted of 4 treatments and 3 replications consisting of treatment K, A, B, and C which mean feeding without cinnamaldehyde, feeding containing cinnamaldehyde dose of 0.05%, 0.1%, and 0.2% respectively. Parameters included total bacteria (green and yellow Vibrio) in water, intestine, and hepatopancreas, as well as immune responses parameters, including total hemocyte count (THC), respiratory burst (RB), and phenol-oxidase (PO). All data were tabulated in Microsoft Excel 2016 and all statistical analysis was conducted in the SPSS v.22.0 software with one-way ANOVA followed by Duncan Multiple Range Test (DMRT (P=0.05). The results showed that the administration of cinnamaldehyde influences the dynamics of Vibrio and health status of shrimp. At the beginning and at the end of cinnamaldehyde administration, green and yellow Vibrio bacteria are mostly found in water, intestine, and hepatopancreas. The administration of cinnamaldehyde to Pacific white shrimp can reduce the number of green Vibrio bacteria both in the intestine and hepatopancreas as shown in treatments C and B. Cinnamaldehyde also affected the higher percentage of yellow Vibrio bacteria in treatments C and B compared to control (K). The highest immune responses of THC and RB were produced in treatments A and C compared to K. The decrease of green Vibrio bacteria and increased colonization of yellow Vibrio bacteria were correlated with immune responses. The best treatment in this study is treatment C.
Effects of Different Commercial Probiotics on Growth and Survival of Oreochromis niloticus Fry Muhammad Arif Mulya; Rifqah Pratiwi; Mohammad Aghistni Rahman; Muthia Khairunnisa
Journal of Vocational in Aquaculture (JAVA) Vol 3 No 1 (2026): MEI 2026
Publisher : College of Vocational Studies, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/bjnstj97

Abstract

Nile tilapia (Oreochromis niloticus) is an important freshwater aquaculture commodity with strong development potential in Indonesia. Increasing market demand has encouraged farmers to improve production efficiency; however, several constraints remain, including aquaculture waste, low feed digestibility, and high feed costs. One approach that has been widely explored is the use of commercial probiotics as feed additives to improve nutrient utilization and overall fish performance. This study aimed to evaluate the effect of different commercial probiotics applied in feed on the growth performance and survival of Nile tilapia fry. The experiment was conducted using a Completely Randomized Design with three treatments and three replications: P0 (feed without probiotics), P1 (feed supplemented with a commercial probiotic containing Bacillus subtilis ≥10⁶ CFU/mL at a dose of 15 mL/kg feed), and P2 (feed supplemented with a commercial probiotic containing Lactobacillus casei ≥10⁶ CFU/mL and Saccharomyces cerevisiae ≥10⁶ CFU/mL at a dose of 20 mL/kg feed). The results showed that treatment P2 produced the best outcomes and differed significantly (P<0.05) from the other treatments. This treatment resulted in higher survival (94.43±7.35%), specific growth rate (8.44±0.30 %/day), absolute weight gain (5.32±0.86 g), and absolute length gain (2.08±0.27 cm/day), along with improved feed conversion ratio (1.26±0.07), feed efficiency (81.34±0.36%), and protein efficiency ratio (8.53±0.36). These findings suggest that the application of the probiotic used in treatment P2 can improve feed utilization and support better growth and survival of Nile tilapia fry under culture conditions.
Selection of local prebiotics to support the growth of probiotic bacteria and fish Dian Eka Ramadhani; Cecilia Eny Indriastuti; M Agung Zaim Adzkiya; Muhammad Arif Mulya; Andri Iskandar; Nida Ulzanah
JURNAL MINA SAINS Vol. 11 No. 2 (2025): Jurnal Mina Sains
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jmss.v11i2.14969

Abstract

Fish production in Indonesia is increasing, but the main obstacle that can hinder the continuity of the cultivation process is disease attacks. Antibiotics are ingredients commonly used to treat fish diseases. However, antibiotics can have negative effects, so other alternatives are needed to overcome this problem. The use of prebiotics in Indonesia still comes from abroad, whereas Indonesia has many ingredients that can be developed into prebiotics. This research aims to identify and select local prebiotic sources that have the potential to meet scientific criteria as probiotics. The research method was carried out in vitro to determine the glucose, maltose, and fructose content in purple sweet potato, banana stem, cream sweet potato, yam tuber and yellow sweet potato. The highest maltose content is produced by purple sweet potatoes (2.61%), fructose content is produced by yellow sweet potatoes (1.80%), and glucose content is produced by creamy sweet potatoes (2.15%). The results of this study need to be further developed into probiotics that can be used to support sustainable aquaculture.  
PERAN SIMPLISIA KUNYIT (Curcuma domestica) DALAM MENEKAN INFEKSI JAMUR PADA LARVA GURAME (Osphronemus gouramy) Andri Iskandar; Miqdad Husamuddin; Yani Hadiroseyani; Muhammad Arif Mulya; Wiyoto Wiyoto; Imam Tri Wahyudi
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 51 No 1 (2026)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v51i1.21304

Abstract

Ikan gurame (Osphronemus gouramy) memiliki nilai ekonomi tinggi, namun sintasan larvanya masih rendah akibat infeksi patogen dan kondisi lingkungan yang sensitif. Penelitian ini bertujuan mengkaji pengaruh perendaman larva ikan gurame dengan menggunakan simplisia kunyit (Curcuma domestica) sebagai agen antimikroba alami untuk meningkatkan kesehatan larva. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri atas tiga perlakuan: kontrol, P1 (30 mg/L simplisia kunyit pada media), dan P2 (30 mg/L pada media serta pakan cacing sutera direndam 1 g/L), masing-masing tiga ulangan. Parameter yang diamati terdiri dari pertumbuhan panjang mutlak, prevalensi jamur, sintasan, dan kualitas air selama empat minggu. Berdasarkan hasil analisis, tidak terdapat perbedaan yang nyata pada pertumbuhan antar perlakuan (P > 0,05). Prevalensi jamur menurun pada P1 (5,16%) dan P2 (4,01%) dibandingkan kontrol (15,71%), menegaskan aktivitas antijamur kunyit. Sintasan berkisar 74,44–82,22% tanpa perbedaan signifikan. Kualitas air tetap dalam kisaran layak. Secara umum, simplisia kunyit efektif menekan jamur tetapi belum meningkatkan pertumbuhan dan sintasan, sehingga diperlukan formulasi yang lebih efisien.
Keragaman dan Distribusi Bakteri Patogen pada Ikan Mas (Cyprinus carpio) di Sentra Budidaya Ikan Mas Pabuaran, Subang, Jawa Barat Andri Iskandar; Ahmad Justine Adillah; Muhammad Arif Mulya; Amalia Putri Firdausi; Mohamad Iqbal Kurniawinata; Wiyoto Wiyoto; Imam Tri Wahyudi; Dian Eka Ramadhani
Jurnal Ilmiah Samudra Akuatika Vol 10 No 1 (2026): Jurnal Ilmiah Samudra Akuatika
Publisher : Fakultas Pertanian Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jisa.v10i1.13991

Abstract

The aquaculture sector frequently encounters disease problems, both infectious and non-infectious. Infectious diseases in fish can be caused by viruses, bacteria, parasites, and fungi, all of which can reduce productivity and threaten the sustainability of aquaculture operations. This study aimed to determine the distribution and types of pathogenic bacteria infecting common carp (Cyprinus carpio) and to analyze the prevalence of these pathogens in common carp farming centers located in Pabuaran and Salam Jaya Villages, Pabuaran District, Subang Regency, West Java. Sampling was conducted using a purposive sampling technique at four observation stations. Bacterial isolation was conducted from kidney and lesion tissues using Tryptic Soy Agar (TSA), followed by identification based on colony morphology, Gram reaction, and biochemical tests including catalase, oxidase, TSIA, MIO, O/F, and glucose fermentation tests. The results showed that in Pabuaran Village, Station 1 contained three types of pathogenic bacteria: Aeromonas sp. (87%), Pseudomonas sp. (6.7%), and Enterobacter sp. (6.7%), while Station 2 contained two types: Aeromonas sp. (93%) and Pseudomonas sp. (7%). In Salam Jaya Village, Station 1 contained Aeromonas sp. (87%) and Pseudomonas sp. (13%), while Station 2 contained Aeromonas sp. (87%) and Enterobacter sp. (13%). Overall, Aeromonas sp. was identified as the dominant pathogenic bacterium infecting common carp in both study locations.
Peningkatan Kinerja Pertumbuhan dan Efisiensi Pakan Juvenil Ikan Gurami Osphronemus gouramy yang Diberi Pakan dengan Suplementasi Enzim Papain dan Probiotik Muhammad Arif Mulya; Muhamad Erlan Hafid Djunaedi; Andri Hendriana; Imam Tri Wahyudi; Cecilia Eny Indriastuti; Andri Iskandar
Vol 15 No 4 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i4.1429

Abstract

Gourami (Osphronemus gouramy) farming faces challenges, including slow growth rates andhigh feed costs. This study aimed to evaluate the effects of probiotics, papain enzymes, andtheir combination on the growth performance and feed utilization of gourami. The study wasconducted using a completely randomized design with four treatments and three replicateseach. The treatments consisted of a control group (feed without papain enzyme or probiotics),treatment A (feed supplemented with 8 mL/kg probiotics), treatment B (feed supplementedwith 32.5 g/kg papain enzyme), and treatment C (feed supplemented with a combination of0.25 g/kg papain enzyme and 15 mL/kg probiotics). Juvenile gourami with average weight of5.82±0.09 g/fish were kept in nine fiberglass tank (3 m diameter and 1.2 m height) for 42 dayswith a stocking density of 15 fish/m2. After 42 days of rearing, treatment C exhibitedsignificantly higher specific growth rate, protein efficiency ratio, and protein retention than theother treatments (P<0.05), with values of 3.03±0.15%/day, 2.78±0.20%, and 44.71±3.06%,respectively. The lowest feed conversion ratio was also observed in treatment C, with a valueof 0.94±0.06 (P<0.05). In conclusion, supplementation with 0.25 g/kg papain enzyme and 15mL/kg probiotics in the feed enhanced growth performance and feed utilization in gourami.
Co-Authors Ahmad Justine Adillah Amalia Putri Firdausi Cecilia Eny Indriastuti Dian Eka Ramadhani Dinamella Wahjuningrum Hendriana, Andri Imam Tri Wahyudi Imam Tri Wahyudi Imam Tri Wahyudi Iskandar, Andri M Agung Zaim Adzkiya Miqdad Husamuddin Mohamad Iqbal Kurniawinata Mohammad Aghistni Rahman Muhamad Erlan Hafid Djunaedi Muthia Khairunnisa Nida Ulzanah Rifqah Pratiwi Wiyoto Wiyoto Wiyoto Wiyoto Y. Hadiroseyani