<![CDATA[This research aims to develop and assess the validity of a differentiated biotechnology e-module as a learning media to enhance high school students' multiliteracy skills. The method used is Research and Development (R&D) with the ADDIE model (Analysis, Design, Development, Implementation, Evaluation). The research stages include needs analysis, e-module design, product development, limited implementation, and validity evaluation. The research subjects were 36 senior high school ten grader science students. The instruments used include validity questionnaires by material experts, media experts, and student response questionnaires. The research results show that the average validity score of the e-module by material experts was 91.96% (very valid category), and by media experts 95.54% (very valid category). Student responses to the e-module showed an average score of 4.7 (very practical category). This data indicates that the developed differentiated biotechnology e-module meets the criteria for high validity and practicality. The implementation of this e-module is expected to enhance students' multiliteracy skills, particularly in digital literacy, science literacy, numeracy literacy, information literacy, as well as cultural and financial literacy, which are important 21st-century competencies. Thus, this e-module can be an effective alternative in biotechnology learning in high school.]]>
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