Optimisasi Enzim Protease oleh Bakteri Endofit dari Akar Tumbuhan Kawasan Ekosistem Karstdengan Response Surface Methodology

Halifah Pagarra (Universitas Negeri Makassar)
Hartati Hartati (Universitas Negeri Makassar)
Rachmawaty Rachmawaty (Universitas Negeri Makassar)

SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam
Vol. 10 No. 2 (2021): Volume 10 Nomor 2 (September 2021)

This study aims to determine the activity of protease enzyme production from bacterial isolate B isolated from the roots of karst plants in various incubation times. The bacterial isolate used was bacterial isolate B, a thermophilic proteolytic bacterium isolated from the Karst Ecosystem in Pangkajene Regency, South Sulawesi Province. Testing of protease activity was carried out using the Lowry method. Highest crude protein. Protein content of all treatments was obtained at 48 hours of incubation for Bacterial Isolate B (5.756 mg/ml).The results showed that Bacterial Isolate B showed optimal production time at 48 hours with enzyme activity 0.393 Unit/ml. It can be concluded that Bacterial Isolate B is a proteolytic bacterium characterized by the formation ofa clear zone around the bacterial colonies in SMA. The production of the protease enzyme obtained by the RSM (CCD) method was optimum at 0.393 U/ml at 0.60% glucose concentration, 0.50% yeast extract and 1.00% skim milk.