Anemia remains a major nutritional problem among adolescent girls and may have long-term consequences if not properly addressed. This study was conducted to assess the effect of combined iron and vitamin C supplementation on hemoglobin levels among adolescent girls. A pre-experimental study with a one-group pretest–posttest design was conducted involving 30 anemic female students at a junior high school in Kintamani, Indonesia. Participants received iron tablets together with vitamin C supplementation, and hemoglobin levels were measured before and after the intervention. Data were analyzed using the Wilcoxon signed-rank test. A significant increase in hemoglobin levels was observed after the intervention. These results indicate that the combination of iron and vitamin C contributes to improved hemoglobin status. The integration of vitamin C into anemia prevention programs, particularly in school-based settings, may enhance the effectiveness of existing interventions. In addition, the use of point-of-care testing is important to support accurate monitoring of hemoglobin levels in resource-limited environments.
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