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All Journal Medical Laboratory Science Journal
Anak Agung Ayu Eka Cahyani
STIKES Wira Medika Bali, Indonesia

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Smoking habits and hemoglobin levels: Implications for donor screening among male blood donors Fernando Rahul Surya Pratama Putra; Diah Prihatiningsih; Anak Agung Ayu Eka Cahyani
Medical Laboratory Science Journal Vol. 1 No. 1 (2025): May 2025
Publisher : Kadewa Bakti Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.67958/medlab.v1i1.25

Abstract

The selection of donors based on smoking habits is an important process to ensure the availability of safe, high-quality blood. This study aims to analyze the correlation between smoking habits and blood hemoglobin levels in men at the Indonesian Red Cross Blood Transfusion Unit in Balikpapan. An analytical observational study with a cross-sectional design was conducted involving 99 male donors who were purposively selected based on their smoking intensity. Hemoglobin levels were measured using a standard hematology analyzer, while smoking habits were evaluated through structured interviews. Data were analyzed using Pearson's correlation test to evaluate the relationship between smoking categories and hemoglobin levels. The results showed a significant positive correlation, with higher smoking intensity associated with increased hemoglobin concentration. These findings confirm the physiological mechanism of compensatory erythropoiesis in smokers and highlight the importance of considering lifestyle factors during donor selection. This study provides insight into the hematological effects of smoking in clinical practice.
Pre–post effect of iron and vitamin C supplementation on hemoglobin in adolescent girls Ni Nyoman Sariani; Ni Luh Gede Puspita Yanti; Anak Agung Ayu Eka Cahyani
Medical Laboratory Science Journal Vol. 2 No. 1 (2026): May 2026
Publisher : Kadewa Bakti Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.67958/medlab.v2i1.78

Abstract

Anemia remains a major nutritional problem among adolescent girls and may have long-term consequences if not properly addressed. This study was conducted to assess the effect of combined iron and vitamin C supplementation on hemoglobin levels among adolescent girls. A pre-experimental study with a one-group pretest–posttest design was conducted involving 30 anemic female students at a junior high school in Kintamani, Indonesia. Participants received iron tablets together with vitamin C supplementation, and hemoglobin levels were measured before and after the intervention. Data were analyzed using the Wilcoxon signed-rank test. A significant increase in hemoglobin levels was observed after the intervention. These results indicate that the combination of iron and vitamin C contributes to improved hemoglobin status. The integration of vitamin C into anemia prevention programs, particularly in school-based settings, may enhance the effectiveness of existing interventions. In addition, the use of point-of-care testing is important to support accurate monitoring of hemoglobin levels in resource-limited environments.
Effect of blood agar storage duration at 2–8°C on bacterial growth from rectal swabs Kadek Ryan Octaviani; Anak Agung Ayu Eka Cahyani; Sri Idayani
Medical Laboratory Science Journal Vol. 2 No. 2 (2026): November 2026
Publisher : Kadewa Bakti Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.67958/medlab.v2i2.96

Abstract

Reliable microbiological testing depends in part on the ability of culture media to support bacterial isolation and the observation of colony characteristics. This study aimed to evaluate differences in bacterial growth from rectal swab specimens stored on Blood Agar Plates at 2–8 °C for varying durations. A laboratory-based experimental study was conducted using 30 Blood Agar Plates divided into three storage-duration groups: 0, 7, and 14 days. Thirty rectal swab specimens were inoculated onto the plates and incubated at 35–37°C for 24 hours. The number of bacterial colonies was assessed as the primary quantitative outcome, while colony size, distribution, color, and hemolytic pattern were evaluated descriptively. Data were analyzed using One-Way ANOVA followed by Tukey's test. The number of bacterial colonies differed significantly among the three storage-duration groups. The highest mean colony count was observed in the 7-day group, while the 14-day group showed the lowest mean count, with significant differences across all pairwise comparisons. The findings indicate a nonlinear relationship between storage duration and bacterial recovery. The substantially lower bacterial recovery after 14 days highlights the importance of controlling the duration of culture medium storage as part of laboratory quality assurance. Further research using standardized bacterial inocula and physicochemical assessments is needed to establish validated storage limits.
Co-Authors Diah Prihatiningsih Fernando Rahul Surya Pratama Putra Kadek Ryan Octaviani Ni Luh Gede Puspita Yanti Ni Nyoman Sariani Sri Idayani