The Anti-Inflammatory Effects of Genistein Inhibits Tumor Necrosis Factor-Α Receptor 1 (TNF-R1) and IL-8 Receptor (CXCR-1) Levels in Peritoneal Endometriosis Lesion of Mice Model Endometriosis

Sutrisno Sutrisno (Department of Obstetric and Ginecology, Faculty of Medicine, Brawijaya University/Saiful Anwar General Hospital, Malang, East Java, Indonesia.)
Cholid Rochman Riskianto (Department of Obstetric and Ginecology, Faculty of Medicine, Brawijaya University/Saiful Anwar General Hospital, Malang, East Java, Indonesia)
Yulianto Rapa (Department of Obstetric and Ginecology, Faculty of Medicine, Brawijaya University/Saiful Anwar General Hospital, Malang, East Java, Indonesia)
Nina Rini Suprobo (Department of Midwifery, Faculty of Medicine, Universitas Negeri Malang, Malang, Indonesia)
Pande Made Dwijayasa (Department of Obstetric and Ginecology, Faculty of Medicine, Brawijaya University/Saiful Anwar General Hospital, Malang, East Java, Indonesia)

Asian Journal of Fertility, Endocrinology, and Reproduction
Vol. 1 No. 1 (2026): January 2026

Introduction: Endometriosis is a complex condition that typically causes an inflammatory reaction in the peritoneal cavity. Tumor Necrosis Factor-α receptor 1 (TNF-R1) and IL-8 receptor (CXCR-1) are thought to play a role in the pathophysiology of endometriosis. This study evaluates the effects of genistein at various doses on the content of TNF-R1 and CXCR1 in the peritoneal endometriosis lesions in mice model of endometriosis. Methods: This experiment study, a total 32 female mice (Mus musculus) with induced endometriosis were allocated to the eight groups (n=4 per group), including a control group, endometriosis model group (EM), and six groups treated with different doses of genistein (0.13, 0.26, 0.52, 0.78, 1.04, and 1.30 mg/day) for 14 days. The peritoneal endometriosis lesion was then measured for TNF-R1 and CXCR-1 levels. Results: The level of TNF-R1 and CXCR-1 in the EM group was significantly elevated compared to the control group. Following genistein administration, TNF-R1 and CXCR-1 levels were significantly lower than in the EM group. Conclusion: Genistein has an anti-inflammatory effect, reducing TNF-R1 and CXCR-1 in mice model of peritoneal endometriosis and could be a potential treatment for endometriosis.