Occupational Health and Safety (OHS) in the operating room is an essential component of healthcare services because healthcare personnel are exposed to various occupational hazards in a high-risk environment. As future healthcare professionals, anesthesiology nursing students are expected to possess adequate knowledge of OHS to ensure safe clinical practice and improve patient safety. This study aimed to describe the level of OHS knowledge among Applied Bachelor of Anesthesiology Nursing students at Universitas Harapan Bangsa based on age and gender. This research employed a descriptive quantitative design with a cross-sectional approach involving 135 respondents selected using the Slovin formula and stratified random sampling technique. Data were collected using a structured questionnaire and analyzed descriptively through frequency and percentage distributions. The results showed that most respondents were 21 years old (51.1%) and female (69.6%). The majority of respondents demonstrated good knowledge of OHS across age and gender categories, and no respondents were classified as having poor knowledge. These findings indicate that Applied Bachelor of Anesthesiology Nursing students at Universitas Harapan Bangsa generally have good knowledge of Occupational Health and Safety in the operating room. Strengthening OHS education through academic learning and clinical practice is expected to further enhance students' competence in implementing safe and professional perioperative care.
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