Improving quality of life is inextricably linked to health puskesmas play a vital role in enhancing the quality of life of the community, making their existence essential. Puskesmas play a role in providing optimal health services to support the community’s quality of life. This study aims to analyze the influence of leadership, work environment, and career development on employee performance through work motivation at the Puskesmas Mlandingan. This study is an explanatory study using a saturation sampling technique among employees of the UPT Puskesmas Mlandingan. Data analysis and hypothesis testing in this study utilized the Partial Least Squares Structural Equation Modeling (PLS-SEM) approach. The results of the direct effect hypothesis test, conducted using the Smart PLS 3.0 software, indicated that leadership has a significant positive effect on work motivation, whereas the work environment has a negative but insignificant effect on work motivation. Career development also showed a significant positive effect on work motivation. In addition, leadership, work environment, and career development each had a positive but insignificant effect on employee performance. Meanwhile, work motivation was also found to have a significant positive effect on employee performance. Regarding indirect effects, leadership had a positive but insignificant effect on employee performance through work motivation, whereas the work environment.
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