<![CDATA[Both chemically and physically, mutation induction is currently being intensively carried out to increase the genetic diversity of porang. However, in-depth evaluation of changes in secondary metabolites after mutation induction has not been widely implemented. Therefore, this study aims to classify the profile of secondary metabolites of porang after colchicine mutation induction and analyze the relationship between these metabolites and the glucomannan synthesis pathway. Porang explants induced with a variation of colchicine concentration were used for profiling metabolite analysis. The metabolite profile was identified using GC-MS (Gas chromatography tandem-mass spectrometry) and then analyzed using MetaboAnalyst. The construction of the metabolite pathway network was carried out using Cytoscape software. 0.02% of colchicine resulted mixoploid porang and has a different metabolite profile than normal porang. In addition, mixoploid porang (0.02% colchicine) has more metabolite content related to glucomannan synthesis. Therefore, mutation induction using a colchicine concentration of 0.02% is recommended to produce superior porang]]>
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