Ruri Siti Resmisari
Department Of Biology, Faculty Of Science And Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang. Jl. Gajayana No. 50 Malang, East Java, Indonesia, 65145

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The Effect of Gibberellic Acid (GA3) On The In Vitro Seed Germination of Manggosteen (Garcinia mangostana) Andini, Andini; Dzaroini, Riska Aqidatud; Resmisari, Ruri Siti
El-Hayah : Jurnal Biologi Vol 6, No 4 (2018): EL-HAYAH (VOL 6, NO 4,March 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i4.6644

Abstract

Mangosteen is considered as fruit of many benefits with great popularity, rendering it valuable as an export commodity. Unfortunately, it has a relatively slow growth  and long early fruiting time. Generative propagation of mangosteen by seed result in low-yielding productionof qualified seedswith recalcitrant nature which means it cannot be stored for a long time. Whereas the result of vegetative propagation using grafting, branch cuttings and budding has difficulty rooting out. Tissue culture method requires growth regulators to control organogenesis and morphogenesis so it can accelerate the growth of the mangosteen explants. This study was arrangedintwo- factor completely randomized design (CRD). The observed variables werethe strength of the medium (½ and 1 MS) and GA3 concentrations (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm and 1 ppm). The variatonson GA3 concentration (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm; 1 ppm) on  different strength MS medium (½ MS and 1 MS) did not significantly affect the growth of radicle and sooth in the  in vitro seeds germination of Mangosteen (Garcinia mangostana).
PENGEMBANGAN BIBIT UNGGUL PORANG (Amarphopallus oncophilus) MELALUI TEKNIK KULTUR IN VITRO UNTUK MENDUKUNG KETAHANAN PANGAN NASIONAL Suheriyanto, Dwi; Romaidi, Romaidi; Resmisari, Ruri Siti
El-Hayah : Jurnal Biologi Vol 3, No 1 (2012): EL-Hayah (Vol 3, No 1, September 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v3i1.2216

Abstract

Porang (Amarphophallus. oncophyllus) is a type of plant tubers that have the potential and prospects for development in Indonesia. In addition to readily available, plants are also capable of producing carbohydrates and high harvest index. The last few years a very large porang needs. Porang plant propagation is vegetative and generative in which to make the seeds are ready to harvest takes between 4-6 months. This study aims to determine kinds of the appropriate medium for growing plants porang network and determine the concentration  of  each  medium,  so  we  get  a  superior  porang  plants.  Research  using factorial completely randomized design. The first factor is a growth regulator BAP concentration: 0 mg / l, 0.5 mg / l, 1.0 mg / l, 1.5 mg / l and 2.0 mg / l, while the second factor is the concentration of IBA 0 mg / l, 0.5 mg / l and 1.0 mg / l, in order to obtain 15 treatments and each treatment made 15 replications. Data were collected for the pengkalusan, contamination callus, callus mortality rate, number of buds, leaf buds height, number of leaves and root. The results showed that the addition of plant growth regulators (hormones) BAP in MS medium effect on the number of shoots, buds and the high number of young leaves. The addition of hormone IBA in MS medium pengkalusan affect the process and the number of roots. The addition of hormone BAP and IBA on MS medium did not significantly affect mortality contamination callus and callus. The interaction between BAP and IBA hormone given on MS medium did not significantly affect the pengkalusan, contamination callus, callus mortality rate, number of buds, leaf buds height, number of leaves and root  
AKTIVITAS ANTIOKSIDAN RAMUAN TRADISIONAL MADURA “SUBUR KANDUNGAN” Resmisari, Ruri Siti
El-Hayah : Jurnal Biologi Vol 5, No 1 (2014): EL-HAYAH (VOL 5, NO 1, September 2014)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i1.3037

Abstract

Traditional medicine "Subur Kandungan" is a medicine that is widely used and produced in Madura. The problem to develop Madura’s herb is raw material that hasn’t the standardized medicine used for Madura’s traditional medicine, because that raw materials are mostly purchased from traditional markets. This led to the standardization of Madura’s traditional medicine is difficult to do. This condition causes didn’t known active compounds contained in the Madura’s medicine. This study was conducted to determine the content of phytochemical compounds from ethanol extract medicine "Subur Kandungan" as well as antioxidant activity. Based on the results, it was concluded that the class of chemical compounds contained in the Madura’s traditional medicine "Subur Kandungan" are classified as a flavonoids and alkaloids, while the antioxidant activity of extracts of the medicine " Subur Kandungan " are expressed in IC50 was at 90.19 ppm classified as a strong antioxidant.
Conservation Of Juwet (Syzygium Cumini) Plant Using In Vitro Culture Techniques Ummah, Maslahatul; Mashluhah, Kholidatul; Resmisari, Ruri Siti
El-Hayah : Jurnal Biologi Vol 7, No 3 (2019): EL-HAYAH (VOL 7, NO 3 SEPTEMBER 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i3.10056

Abstract

Juwet (Syzygium cumini)  is a plant that has benefits as a medicine for type II diabetes mellitus, lungs, coughing, laxative urine. However, juwet is a scarce plant, it needs cultivation as a form of cuonservation. One of them is by utilizing biotechnology, which is of plant tissue culture. This study aims to determine the effect of the combination of 2,4-D and BAP growth regulators on the growth of juwet embryonic callus and the effect of the combination NAA and BAP on the growth of axillary bud juwet as a conservation effort. This research is experimental. Using a Completely Randomized Design (RAL) with combination 2,4-D (0; 0.5; 1; 1.5; 2; 2.5; 5) mg/L, and BAP (0; 0.25; 0.5; 0.75; 1) mg/L and combination NAA (0; 0.25; 0.5; 0.75; 1) mg/L and BAP (0; 0.5; 1; 1.5; 2) mg/L. Analysis by Two Way ANAVA test α = 5%. If there is a significant difference, the Duncan Multiple Range Test (DMRT) test with a significant level of 5%. Addition of 2.5 mg/L and 3 mg/L 2.4-D without BAP can induce intermediate callus, brownish yellow and there is a large cell nucleus in each cell. While the results of axillary bud growth is treatment in BAP 1 mg/L without NAA is the most effective interaction on the emergence of buds is 26.6 days after planting with the total of buds as much as 6.66, and the highest buds 5.37 cm and the highest total of leaves, namely 8.33 strands. 
Phytochemistry Screening and Antioxidant Activities of Extract Pomegranate, Grape, Fig, and Olive in the Various Solvent Evika Sandi Savitri; Kholifah Holil; Ruri Siti Resmisari
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.13424

Abstract

The active compounds of grape, pomegranate, olive, and fig have anthocyanins that potential as antioxidant are flavonoids. Flavonoids have potential as antioxidant  to prevent and therapy various oxidative stress and related diseases. This study aimed to examine the antioxidant activity of a combination of pomegranate, grape, fig, and olive extracts using the DPPH (diphenyl-2-picrylhydrazyl) method.  The maceration method used was maceration of dry Simplicia with methanol 95% solvent, fresh maceration with 95% methanol and dry Simplicia with 95% ethanol solvent. The results of the phytochemistry test showed several compounds found in the extract combination pomegranate, grapes, fig, and olives such as polyphenol, flavonoid, tannin, steroid/triterpenoid. The result of the antioxidant test showed the fresh maceration 95% methanol showed higher results with the IC50 of 25.22 with a potent antioxidant activity category.
Schleichera oleosa (Molk.)Oken Callus Induced BAP and 2,4 D In vitro Ruri Siti Resmisari; Azizatur Rahmah
El-Hayah Vol 8, No 4 (2022): EL-HAYAH (VOL 8 NO.4 MARET 2022)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v8i4.15719

Abstract

Schleichera oleosa (Lour.) Oken is a plant that it can used as drug candidate. S. oleosa has several health benefits including as anti-cancer, anti-oxidant, and anti-microbial. One of technique for increasing the secondary metabolite in S. oleosa (Lour.) Oken is callus culture. This study is an experimental research using Factorial Completely Randomized Design (CRD). This study consist of 2 factors are  Benzyl Amino Purin (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) with various concentrations. Based on the result of callus induction of S. oleosa (Lour.) Oken, it gives some different effect for the variable. The day of emerging callus and weight of callus give the optimum result at 2 mg/L 2,4-D + 0,5 mg/L BAP for 32 Day After Planting and 1,1458 grams. Besides, the percentage of callus formation at 1 mg/L 2,4-D + 0,5 mg/L BAP for 83,33%. 
Karakter Anatomi KesambiI (Schleichera oleosa (Lour) Oken.,) Pada Ketinggian Yang Berbeda Di Kota Malang Dan Kabupaten Nganjuk Ruri Siti Resmisari; Azizatur Rahmah; Kholifah Holil; Tias Pramesti Griana
Metamorfosa: Journal of Biological Sciences Vol 10 No 2 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i02.p06

Abstract

Indonesia dikenal sebagai negara yang memiliki keanekaragaman hayati yang cukup tinggi, namun data yang ada masih sangat terbatas. Satu diantara kenekaragaman hayati yang dimiliki oleh Indonesia adalah Kesambi (Schleichera oleosa). Tanaman ini memiliki potensi yang cukup besar, karena itu dibutuhkan data yang mendukung untuk pengembangan lebih lanjut baik untuk kepentingan sebagai tanaman obat maupun untuk yang lain. Penelitian identifikasi karakter anatomi bertujuan untuk mengetahui sifat-sifat penting dari spesies tanaman yang berasal dari berbagai habitat tumbuh. Karakter anatomi daun yang diamati meliputi ketebalan xylem dan floem, ketebalan kutikula, ketebalan epidermis, tipe trikoma, rata-rata panjang trikoma, tipe stomata, panjang dan lebar stomata. Berdasarkan hasil penelitian dapat disimpulkan bahwa Pohon Kesambi yang tumbuh baik didataran rendah maupun tinggi di Kota Malang dan di Kabupaten Nganjuk memiliki karakter anatomi yang sama yaitu pada tipe trikoma dan stomata. Sedangkan pada karakter lain menunjukkan perbedaan pada ukuran. Semakin tinggi tempat tumbuh pohon kesambi menunjukkan semakin tipis floem maupun xylem pada batang yang diamati. Sedangkan pada parameter lain tidak menunjukkan pola yang terbentuk pada ukuran karakter yang diamati baik pada daun maupun batang. Kata kunci: Anatomi, Kesambi (Schleichera oleosa), faktor lingkungan
Sosialisasi Pembuatan Makanan dan Minuman Halal Toyyib Berbasis Bahan Pangan Alami di Kelurahan Merjosari, Lowokwaru, Malang Azizatur Rahmah; Kholifah Holil; Ruri Siti Resmisari; Tias Pramesti Griana; Umaiyatus Syarifah
Jurnal SOLMA Vol. 13 No. 1 (2024)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v13i1.13100

Abstract

Thoyyiban halal food is a fundamental requirement for the Muslim community in terms of fulfilling nutritional needs. The significance of halal and thoyyiban food lies not only in its role as a source of nutrition but also in its contribution to mental and spiritual well-being. Presently, there is a pressing issue within the community concerning the lack of attention during the selection of processing materials and procurement efforts. Natural ingredients serve as an alternative to address this issue. Consequently, community empowerment aims to socialize and provide training in the production of food based on halal and thoyyiban principles using natural ingredients in the Merjosari village, Malang. The type of community service carried out involves engaging the community not only as the object but also as the subject of the community service. The participants in this activity include the representatives of the Women's Welfare Movement (PKK) from sub-districts 3 and 4 of Merjosari, Lowokwaru, Malang. The community members are provided with socialization and training regarding the production of food based on halal and thoyyiban principles using natural ingredients. The results of this community service activity demonstrate a positive response from the participants. Data indicates a good level of understanding among the participants regarding the content and practices, amounting to 75%. This is evidenced by the participants' ability to select halal and thoyyiban ingredients and create flavor enhancer products using shrimp shells and health drinks
The Effect of 2,4-D (2,4-Dichlorophenoxyacetic Acid) and Kinetin (6-Furfuryl Amino Purine) Concentrations on The Induction of Embryogenic Callus In Porang (Amorphophallus muelleri Blume) In Vitro Agung, Nadya Rahadianti; Huddi, Darul; Putri, Amalia Nasyira; Resmisari, Ruri Siti
El-Hayah:Jurnal Biologi Vol 9, No 2 (2023): EL-HAYAH (VOL 9, NO 2 Maret 2023)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i2.26105

Abstract

Porang (Amorphophallus muelleri Blume) belongs to the family Araceae and holds significant potential for cultivation in Indonesia. It contains more than 50% glucomannan in its corms. Porang is commonly commercialized as flour and chips and has found extensive use in the food, cosmetics, chemical, pharmaceutical, and coating industries. Conventional propagation of Porang often takes a long time, approximately 2-3 years, making it unable to meet the demand for Porang seedlings quickly. In vitro propagation using plant growth regulators 2,4-D and kinetin is being explored to induce embryogenic callus, offering a solution for large-scale cultivation of high-quality Porang within a shorter timeframe. This research adopts an experimental approach employing a completely randomized design (CRD) with two factors. The first factor is the auxin 2,4-D (2,4-Dichlorophenoxyacetic acid), and the second factor is the cytokinin kinetin. The optimal concentration for inducing embryogenic callus in Porang is found to be 2,4-D at 1 mg/L and kinetin at 0 mg/L, resulting in intermediate callus with a yellowish-brown color and a wet weight of 2.43 grams.
Induction of Synthetic Polyploids of Porang (Amorphophallus muelerri Blume) and Assessment of Its Genetic Variability Using Morphological Data and RAPD Molecular Marker Suyono, Suyono; Indivia, Imey Tamara; Resmisari, Ruri Siti; Fitriyah, Fitriyah; Wahyudi, Didik
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82238

Abstract

This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation.